Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis.
Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis.
复制标题
拟南芥中可剪接 5 至 10 公斤碱基对的大内含子
DOI：
10.3390/genes8080200
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发表时间：
2017-08-11
期刊：
影响因子：
3.5
通讯作者：
Gao AH
中科院分区：
文献类型：
--
作者：
Chang N;Sun Q;Hu J;An C;Gao AH
大多数真核基因含有内含子，其在RNA加工过程中从前体RNA中去除。与动物中通常为几千个碱基对（kb）的内含子相比，植物中的内含子通常非常小，大多数为几十个碱基对（bp）至几百个bp。根据拟南芥基因组注释版本10.0，核基因中有127，854个内含子;其中99.23%小于1 kb，只有16个内含子被注释为大于5 kb，这是拟南芥中的极大内含子（ELI）。为了了解这些内含子是否是真正的内含子，以及在拟南芥中内含子可能有多大，对含有这些ELI的基因进行了RT-PCR分析。结果表明，这些推定的内含子中的一些确实是ELI。这些ELI主要由转座子或转座因子（TE）组成，除了一个，其对应物在不同的植物物种中也很长。因此，本研究证实了拟南芥中存在大于5 kb甚至10 kb的内含子。
Most of the eukaryotic genes contain introns, which are removed from the pre-RNA during RNA processing. In contrast to the introns in animals, which are usually several kilo base pairs (kb), those in plants generally are very small, which are mostly from dozens of base pairs (bp) to a few hundred bp. According to annotation version 10.0 of the genome of Arabidopsis thaliana, there are 127,854 introns in the nuclear genes; 99.23% of them are less than 1 kb, and only 16 introns are annotated to be larger than 5 kb, which are extremely large introns (ELI) in Arabidopsis. To learn whether these introns are true introns or not and how large introns could be in Arabidopsis, RT-PCR analysis of genes containing these ELIs were carried out. The results indicated that some of these putative introns are indeed ELIs. These ELIs are mainly composed of transposons or transposable elements (TE), excepting one, whose counterparts are also very long in diverse plant species. Thus, this study confirms the existence of introns larger than 5 kb or even 10 kb in Arabidopsis.
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