9.3 KDa components of the injected venom of Conus purpurascens define a new five-disulfide conotoxin framework.
9.3 KDa components of the injected venom of Conus purpurascens define a new five-disulfide conotoxin framework.
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DOI：
10.1002/bip.21406
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发表时间：
2011
期刊：
影响因子：
2.9
通讯作者：
Mari, Frank
中科院分区：
文献类型：
--
作者：
Moeller, Carolina;Mari, Frank
关键词：
从紫锥芋螺（Conus purpurascens）毒液中分离得到83个残基的芋螺肽（p21 a）及其相应的非羟基化类似物。完整的芋螺肽序列来自胰蛋白酶、胰凝乳蛋白酶和溴化氰双酶片段的Edman降解测序。p21 a具有独特的10-胱氨酸/5-二硫键7-环框架，分别具有延长的10-残基N-末端和5-残基C-末端尾部。p21 a与最近描述的13-胱氨酸芋螺肽con-ikot-ikot具有48%的序列同源性，con-ikot-ikot分离自猎鱼蜗牛Conus striatus的解剖毒液。然而，与con-ikot-ikot不同，p21 a不形成二聚体的二聚体。MALDI-TOF质谱表明p21 a可能形成非共价二聚体。p21 a是一种非常大的芋螺毒素，并且是从注射毒液中分离的最大的芋螺毒素。p21 a提供的证据表明，芋螺毒液库包括直接注入猎物的较大分子。因此，锥螺可以利用与其他有毒动物中常见的毒素大小相当的毒素。
The 83-residue conopeptide (p21a) and its corresponding non-hydroxylated analog were isolated from the injected venom of Conus purpurascens. The complete conopeptide sequences were derived from Edman degradation sequencing of fragments from tryptic, chymotryptic and cyanogen bromide digestions. p21a has a unique, ten-cystine/5-disulfide 7-loop framework with extended 10-residue N-terminus and a 5-residue C-terminus tails, respectively. p21a has a 48% sequence homology with a recently described 13-cystine conopeptide, con-ikot-ikot, isolated from the dissected venom of the fish-hunting snail Conus striatus. However, unlike con-ikot-ikot, p21a does not form a dimer of dimers. MALDI-TOF mass spectrometry suggests that p21a may form a non-covalent dimer. p21a is an unusually large conotoxin and insofar is the largest isolated from injected venom. p21a provides evidence that the Conus venom arsenal includes larger molecules that are directly injected into the prey. Therefore, cone snails can utilized toxins that are comparable in size to the ones commonly found in other venomous animals.
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DOI：
10.1007/978-3-540-30880-5_4
发表时间：
2006-01-01
期刊：
MOLLUSCS: FROM CHEM-ECOLOGICAL STUDY TO BIOTECHNOLOGICAL APPLICATION
影响因子：
--
作者：
Franco, A.;Pisarewicz, K.;Mari, F.
通讯作者：
Mari, F.
影响因子：
4.7
作者：
Jimenez, EC;Shetty, RP;Olivera, BM
通讯作者：
Olivera, BM
影响因子：
2.9
作者：
OLIVERA, BM;MCINTOSH, JM;GRAY, WR
通讯作者：
GRAY, WR
影响因子：
4.8
作者：
Osipov, Alexey V.;Kasheverov, Igor E.;Utkin, Yuri N.
通讯作者：
Utkin, Yuri N.
影响因子：
3.3
作者：
Quinton, Loic;Gilles, Nicolas;De Pauw, Edwin
通讯作者：
De Pauw, Edwin