Transforming growth factor beta modulates proliferation of osteoblastic cells: relation to its effect on receptor levels for epidermal growth factor.
Transforming growth factor beta modulates proliferation of osteoblastic cells: relation to its effect on receptor levels for epidermal growth factor.
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转化生长因子β调节成骨细胞的增殖：与其对表皮生长因子受体水平的影响有关。
DOI：
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发表时间：
1989
影响因子：
6.2
通讯作者：
J. Konishi
中科院分区：
文献类型：
--
作者：
S. Uneno;I. Yamamoto;T. Yamamuro;H. Okumura;S. Ohta;K. Lee;R. Kasai;J. Konishi
研究转化生长因子-β(TGF-β)对成骨细胞MC3T3-E1细胞增殖的影响，重点研究其对表皮生长因子(EGF)受体的调节作用。据报道，在其他细胞中，转化生长因子-β可以增强EGF受体。MC3T3-E1细胞暴露于转化生长因子-β后，细胞表面EGF受体水平增加，DNA合成速率降低。最初EGF受体水平的升高是由于每个细胞的受体数量增加，而不是由于结合亲和力的变化。相反，长时间暴露(超过40h)会导致EGF受体减少，DNA合成速率增加。因此，转化生长因子-β对这些细胞的作用是双相的，反映了复杂的作用机制；转化生长因子-β的早期作用可能与细胞向成骨细胞表型分化一致，细胞增殖减少，而长期暴露于转化生长因子-β刺激细胞增殖，抑制成骨细胞表型表达。由于转化生长因子-β并不能增强EGF对细胞增殖的刺激作用，因此不可能通过提高EGF受体水平来刺激细胞增殖。因此，我们认为，转化生长因子-β对成骨细胞MC3T3-E1细胞的增殖具有促进作用，其作用不依赖于其对EGF受体水平的调节作用。
The effect of transforming growth factor beta (TGF-beta) on cellular proliferation of osteoblastic MC3T3-E1 cells was studied with particular emphasis on its effect on modulation of epidermal growth factor (EGF) receptors. In other cells, TGF-beta has been reported to augment EGF receptors. Exposure of MC3T3-E1 cells to TGF-beta initially increased cell surface EGF receptor levels and decreased the rate of DNA synthesis. The initial elevation of EGF receptor levels was due to increased receptor number per cell, not to changes in binding affinity. On the contrary, prolonged exposure (longer than 40 h) resulted in a decrease in EGF receptor and an increase in the rate of DNA synthesis. Thus, the effects of TGF-beta on these cells appears to be biphasic, reflecting complex mechanisms of action; the early effects of TGF-beta may be consistent with cellular differentiation to the osteoblastic phenotype with decreased cellular proliferation, whereas chronic exposure of these cells to TGF-beta stimulated cellular proliferation and inhibited osteoblastic phenotype expression. It is not likely that stimulation of cellular proliferation was through elevation of EGF receptor levels, because TGF-beta did not enhance the stimulatory effect of EGF on cellular proliferation. Thus, we conclude that TGF-beta possesses a stimulatory effect on the cellular proliferation of osteoblastic MC3T3-E1 cells independent of its modulative effect on EGF receptor level.
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α 和 β 人类转化生长因子刺激培养小鼠颅骨中前列腺素的产生和骨吸收。
DOI：
10.1073/pnas.82.13.4535
发表时间：
1985
影响因子：
11.1
作者：
TashjianJr,AH;Voelkel,EF;Lazzaro,M;Singer,FR;Roberts,AB;Derynck,R;Winkler,ME;Levine,L
通讯作者：
Levine,L
DOI：
10.1016/s0021-9258(18)61587-x
发表时间：
1987-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Centrella;T. McCarthy;E. Canalis
通讯作者：
M. Centrella;T. McCarthy;E. Canalis
转化和非转化生长因子存在于由胎鼠颅盖调节的培养基中。
DOI：
10.1073/pnas.82.21.7335
发表时间：
1985
影响因子：
11.1
作者：
Centrella,M;Canalis,E
通讯作者：
Canalis,E
DOI：
10.1073/pnas.84.7.2024
发表时间：
1987-04-01
影响因子：
11.1
作者：
PFEILSCHIFTER, J;MUNDY, GR
通讯作者：
MUNDY, GR
人血小板衍生的转化生长因子-β 刺激胎鼠颅骨的骨生长参数。
影响因子：
4.8
作者：
Centrella,M;Massague,J;Canalis,E
通讯作者：
Canalis,E