Urinary microbiota of women with recurrent urinary tract infection: collection and culture methods.
Urinary microbiota of women with recurrent urinary tract infection: collection and culture methods.
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DOI：
10.1007/s00192-021-04780-4
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发表时间：
2022-03
影响因子：
1.8
通讯作者：
Brubaker L
中科院分区：
文献类型：
--
作者：
Hochstedler BR;Burnett L;Price TK;Jung C;Wolfe AJ;Brubaker L
许多临床医生利用标准的尿液培养来指导女性复发性尿路感染（鲁蒂）的治疗。然而，尽管抗生素治疗，症状可能持续存在，事件经常复发。鲁蒂的周期性和无效治疗表明，由于大肠杆菌的优先生长，潜在的泌尿道病原体未被检测到。扩大定量尿培养（EQUC）检测更多的临床相关微生物。本研究的目的是评估尿液收集和培养方法如何影响鲁蒂感染患者的微生物检测。这项横断面研究招募了确诊为鲁蒂感染的有症状的成年女性。参与者通过标准尿培养（SUC）和EQUC提供中段排尿和导尿尿液标本进行培养。在培养和收集方法之间比较微生物群的存在和丰度。43名有症状的女性参与者（平均年龄67岁）提供了标本。与SUC相比，EQUC检测到更多独特的细菌种类，并始终从导尿和排尿标本中检测到更多的尿路病原体。对于两种采集方法，EQUC最常检测到的尿路病原体是E。大肠埃希菌（插管8例，排空12例）;粪便（插管：n=7，排尿：n=17）。与EQUC评估的导尿管尿液样本相比，SUC经常遗漏尿路病原体，并且通过任一方法进行排尿培养产生高假阳性率。在有症状的鲁蒂女性中，SUC和排尿评估在尿路病原体检测中具有临床相关局限性。这些结果表明，在该人群中，通过EQUC分析的插管标本为适当的诊断提供了临床相关信息。在有症状的鲁蒂感染妇女中，当使用扩展的定量尿培养分析导管尿液样本时，临床医生获得最临床相关的信息。
Many clinicians utilize standard culture of voided urine to guide treatment for women with recurrent urinary tract infections (RUTI). However, despite antibiotic treatment, symptoms may persist and events frequently recur. The cyclic nature and ineffective treatment of RUTI suggests that underlying uropathogens pass undetected due to the preferential growth of Escherichia coli. Expanded quantitative urine culture (EQUC) detects more clinically relevant microbes. The objective of this study was to assess how urine collection and culture methods influence microbial detection in RUTI patients. This cross-sectional study enrolled symptomatic adult women with an established RUTI diagnosis. Participants contributed both midstream voided and catheterized urine specimens for culture via both standard urine culture (SUC) and EQUC. Presence and abundance of microbiota were compared between culture and collection methods. 43 symptomatic women participants (mean age 67 years) contributed specimens. Compared to SUC, EQUC detected more unique bacterial species and consistently detected more uropathogens from catheterized and voided urine specimens. For both collection methods, the most commonly detected uropathogens by EQUC were E. coli (catheterized: n=8, voided: n=12) and E. faecalis (catheterized: n=7, voided: n=17). Compared to catheterized urine samples assessed by EQUC, SUC often missed uropathogens and culture of voided urines by either method yielded high false positive rates. In women with symptomatic RUTI, SUC and assessment of voided urines has clinically relevant limitations in uropathogen detection. These results suggest that, in this population, catheterized specimens analyzed via EQUC provides clinically relevant information for appropriate diagnosis. In women with symptomatic RUTI, clinicians receive the most clinically relevant information when a catheterized urine sample is analyzed using expanded quantitative urine culture.
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影响因子：
--
作者：
KASS, EH
通讯作者：
KASS, EH
DOI：
10.1017/s0963548311000332
发表时间：
--
期刊：
COMBINATORICS PROBABILITY & COMPUTING
影响因子：
0.79
作者：
Alain Plagne
通讯作者：
Alain Plagne
影响因子：
5.2
作者：
Hirakawa, Hidetada;Suzue, Kazutomo;Tomita, Haruyoshi
通讯作者：
Tomita, Haruyoshi
影响因子：
1.8
作者：
Price, Travis K.;Hilt, Evann E.;Brubaker, Linda
通讯作者：
Brubaker, Linda
DOI：
10.1007/s10096-013-1935-4
发表时间：
2014-01-01
影响因子：
4.5
作者：
Tapiainen, T.;Hanni, A. -M.;Uhari, M.
通讯作者：
Uhari, M.