Cyclic‐AMP inhibits cell growth and negatively interacts with platelet membrane glycoprotein expression on the Dami human megakaryoblastic cell line
Cyclic‐AMP inhibits cell growth and negatively interacts with platelet membrane glycoprotein expression on the Dami human megakaryoblastic cell line
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环-AMP 抑制细胞生长，并与 Dami 人巨核细胞系上的血小板膜糖蛋白表达产生负相互作用
DOI：
10.1002/jcp.1041630327
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发表时间：
1995
影响因子：
5.6
通讯作者：
C. Chevillard
中科院分区：
文献类型：
--
作者：
D. Vittet;C. Duperray;C. Chevillard
Intracellular signaling processes by which hematopoietic growth factors regulate megakaryocytopoiesis remain uncompletely understood. Cyclic AMP (cAMP) has been shown to be implicated in the regulation of growth and differentiation in various normal and malignant cell types. Since a few studies have suggested the possible involvement of the cAMP pathway as one of the intracellular mechanisms whereby megakaryocytopoiesis may be regulated, we investigated the functional effects of cAMP on the human megakaryoblastic Dami cell line. We observed that exposure of Dami cells to cAMP analogs or to agents elevating intracellular cAMP levels yielded dose‐dependent cell growth inhibition. Cell cycle progression analysis of cells predominantly synchronized at the G1/S boundary by prior treatment with hydroxyurea revealed that cAMP transiently accumulated cells in the G2/M phase, then slowing down cell cycle. On the other hand, immunofluorescence and Northern blot analysis of megakaryocytic differentiation marker expression showed that probes we have used significantly inhibited GPlb expression. Moreover, although these agents used alone did not affect GPllb/llla expression, they markedly reversed phorbol ester‐induced GPllb/llla expression increase. These inhibitory cAMP actions on glycoprotein expression were not the result of cell cycle perturbation since we observed that GPlb and GPllb/llla expression were not cell cycle dependent. All these data may then be consistent with a potential negative regulatory role of the cAMP intracellular signaling pathway during megakaryocytopoiesis. © 1995 Wiley‐Liss, Inc.
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DOI：
10.1126/science.6267695
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Martin,TF;Kowalchyk,JA
通讯作者：
Kowalchyk,JA
DOI：
10.1073/pnas.90.21.10300
发表时间：
1993-11-01
影响因子：
11.1
作者：
GRAVES, LM;BORNFELDT, KE;KREBS, EG
通讯作者：
KREBS, EG
影响因子：
20.3
作者：
S. Greenberg;Rosenthal Ds;T. A. Greeley;R. Tantravahi;R. Handin
通讯作者：
S. Greenberg;Rosenthal Ds;T. A. Greeley;R. Tantravahi;R. Handin
影响因子：
3.6
作者：
I. Feoktistov;Italo Biaggioni
通讯作者：
I. Feoktistov;Italo Biaggioni
DOI：
10.1016/s0021-9258(18)82362-6
发表时间：
1993-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Davis
通讯作者：
R. Davis