GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean.

GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean.
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GmBIC 调节低蓝光诱导的大豆茎伸长

DOI:
10.3389/fpls.2022.803122
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发表时间:
2022
影响因子:
5.6
通讯作者:
Liu B
Liu B
中科院分区:
生物学2区
文献类型:
--
作者:
Mu R;Lyu X;Ji R;Liu J;Zhao T;Li H;Liu B

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蓝光隐花色素抑制剂(BICs)通过抑制拟南芥中隐花色素的活性促进下胚轴伸长。然而,BICs在其他植物物种中的作用仍然不清楚。在这里,我们通过基因过表达和CRISPR/Cas9工程突变来研究它们在大豆中的功能。我们发现GmBICs过度表达(GmBICs-ox)系强烈地促进了茎的伸长,而GmBIC基因的单、双和四重突变导致了增加的矮化表型。此外,GmBIC2a的过表达取消了低蓝光(LBL)诱导的茎伸长,表明GmBICs参与了隐色素介导的LBL诱导的遮荫回避综合征(SAS)的调节。四重突变体Gmbi 1a1b2a2b在LbL条件下茎伸长降低,这与GmCRY1b-OX系相似。综上所述,本研究为进一步阐明GmBICs的作用机制和培育耐荫大豆品种提供了必要的遗传资源。
Blue-light inhibitors of cryptochromes (BICs) promote hypocotyl elongation by suppressing the activity of cryptochromes in Arabidopsis. Nevertheless, the roles of BICs in other plant species are still unclear. Here we investigate their functions by genetic overexpression and CRISPR/Cas9 engineered mutations targeting the six GmBIC genes in soybean. We showed that the GmBICs overexpression (GmBICs-OX) lines strongly promoted stem elongation, while the single, double, and quadruple mutations in the GmBIC genes resulted in incremental dwarfing phenotypes. Furthermore, overexpression of GmBIC2a abolished the low blue light (LBL)-induced stem elongation, demonstrating the involvement of GmBICs in regulating cryptochrome-mediated LBL-induced shade avoidance syndrome (SAS). The Gmbic1a1b2a2b quadruple mutant displayed reduced stem elongation under LBL conditions, which was reminiscent of the GmCRY1b-OX lines. Taken together, this study provided essential genetic resources for elucidating GmBICs functional mechanisms and breeding of shade-tolerant soybean cultivars in future.
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