CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites.
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites.
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DOI：
10.1093/bioinformatics/btu743
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发表时间：
2015-04-01
期刊：
影响因子：
--
通讯作者：
Ui-Tei K
中科院分区：
文献类型：
--
作者：
Naito Y;Hino K;Bono H;Ui-Tei K
摘要：CRISPRDirect是一个简单且功能强大的Web服务器，用于从输入序列中选择Rational CRISPR/CAS目标。CRISPR/Cas系统是一种很有前途的基因组工程技术，它允许在Cas9核酸酶的引导下，通过与∼20个碱基的靶序列互补结合的引导核糖核酸(GRNA)，对基因组DNA进行靶向切割。目标序列要求是双重的。首先，5‘-NGG Protspacer邻近基序(PAM)序列必须与靶序列相邻。其次，目标序列应该在整个基因组中是特定的，以避免非目标编辑。CRISPRDirect使用户能够通过对基因组序列进行详尽的搜索，轻松地选择具有最小非靶点的合理靶标序列。该服务器目前整合了人类、小鼠、大鼠、绒猴、猪、鸡、青蛙、斑马鱼、芝麻、果蝇、家蚕、秀丽线虫、拟南芥、水稻、高粱和萌芽酵母的基因组序列。可用性：可在http://crispr.dbcls.jp/.上免费获得联系方式：y-neito@dbcls.rois.ac.jp补充信息：补充数据可在BioInformation Online上获得。
Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ∼20 nt targeted sequence. The target sequence requirements are twofold. First, the 5′-NGG protospacer adjacent motif (PAM) sequence must be located adjacent to the target sequence. Second, the target sequence should be specific within the entire genome in order to avoid off-target editing. CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic sequences. The server currently incorporates the genomic sequences of human, mouse, rat, marmoset, pig, chicken, frog, zebrafish, Ciona, fruit fly, silkworm, Caenorhabditis elegans, Arabidopsis, rice, Sorghum and budding yeast. Availability: Freely available at http://crispr.dbcls.jp/. Contact: y-naito@dbcls.rois.ac.jp Supplementary information: Supplementary data are available at Bioinformatics online.
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