The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells.
The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells.
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DOI：
10.1007/s10719-008-9207-8
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发表时间：
2009-07
影响因子：
3
通讯作者：
Fukuda, Minoru
中科院分区：
文献类型：
--
作者：
Mitoma, Junya;Miyazaki, Tatsuo;Sutton-Smith, Mark;Suzuki, Misa;Saito, Hideo;Yeh, Jiunn-Chern;Kawano, Takehiro;Hindsgaul, Ole;Seeberger, Peter H.;Panico, Maria;Haslam, Stuart M.;Morris, Howard R.;Cummings, Richard D.;Dell, Anne;Fukuda, Minoru
E-， P-和l -选择素在淋巴细胞再循环和将白细胞募集到炎症部位中起关键作用。MECA-79抗体抑制多种物种中l -选择素介导的淋巴细胞粘附，其表位不需要唾液酸。然而，许多其他抗体可以识别表达n -乙酰神经氨酸的人类选择素配体，而不能识别表达n -糖基神经氨酸的小鼠选择素配体，这表明唾液酸的差异导致了唾液酸Lewis X的不同反应性。我们发现，hea -452和FH6单克隆抗体结合了表达n -乙酰神经胺基Lewis X寡糖的中国仓鼠卵巢（CHO）细胞，而不是其n -乙醇基形式。此外，合成的n -乙酰神经胺基Lewis X寡糖抑制hea -452与FH6的结合，而其n -乙醇基寡糖抑制不了hea -452与FH6的结合。相比之下，E-、P-和l -选择素与CHO细胞结合，无论它们表达的是n -乙酰基还是n -乙醇基形式的唾液酰Lewis X，这表明选择素比HECA-452和FH-6抗唾液酰Lewis X抗体具有更广泛的识别能力。
E-, P- and L-selectins critically function in lymphocyte recirculation and recruiting leukocytes to inflammatory sites. MECA-79 antibody inhibits L-selectin-mediated lymphocyte adhesion in several species and does not require sialic acid in its epitope. Many other antibodies, however, recognize human selectin ligands expressing N-acetylneuraminic acid but not mouse selectin ligands expressing N-glycolylneuraminic acid, suggesting that difference in sialic acid in sialyl Lewis X leads to differential reactivity. We found that HECA-452 and FH6 monoclonal antibodies bind Chinese hamster ovary (CHO) cells expressing N-acetylneuraminyl Lewis X oligosaccharide but not its N-glycolyl form. Moreover, synthetic N-acetylneuraminyl Lewis X oligosaccharide but not its N-glycolyl oligosaccharide inhibited HECA-452 and FH6 binding. By contrast, E-, P- and L-selectin bound to CHO cells regardless of whether they express N-acetyl or N-glycolyl form of sialyl Lewis X, showing that selectins have a broader recognition capacity than HECA-452 and FH-6 anti-sialyl Lewis x antibodies.
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影响因子：
56.9
作者：
Butcher, EC;Picker, LJ
通讯作者：
Picker, LJ
影响因子：
4.8
作者：
Hiraoka, N;Kawashima, H;Fukuda, M
通讯作者：
Fukuda, M
影响因子：
82.9
作者：
Dagia, Nilesh M.;Gadhoum, Samah Z.;Sackstein, Robert
通讯作者：
Sackstein, Robert
影响因子：
3
作者：
ITO, K;HANDA, K;HAKOMORI, SI
通讯作者：
HAKOMORI, SI
影响因子：
30.5
作者：
Kawashima, H;Petryniak, B;Fukuda, M
通讯作者：
Fukuda, M