Control of the onset of migration of neural crest cells in avian embryos
Control of the onset of migration of neural crest cells in avian embryos
复制标题
控制禽类胚胎中神经嵴细胞迁移的开始
作者：
Donald F. Newgreen;D. Gooday
为了研究神经嵴上皮-间充质转化为迁移群体的时间控制，在Ca+ +存在下通过蛋白酶从2天鹌鹑胚胎中分离神经原基（神经管加嵴），并将其固定在有利于神经嵴细胞迁移的基质上。在正常迁移开始前分离的外植体在体外需要3-8小时，神经嵴细胞开始迁移，但在迁移阶段获得的外植体显示迁移立即开始。时间表与体内预期相似。当迁移前的神经原基分离蛋白酶在Ca+ +和Mg+ +-免费（CMF）介质中，或当蛋白酶随后是一个简短的（5分钟）暴露于CMF介质，神经嵴细胞迁移开始没有延迟，和原基的凝聚力受损。无Ca+ +培养基复制CMF的效果，但无论是Mg+ +无培养基，也没有CMF处理前与蛋白酶刺激迁移和降低凝聚力。迁移前神经原基与膜共培养时，神经嵴细胞迁移早，细胞内聚性降低。Ca+ +-通道拮抗剂D 600和硝苯地平，而没有任何外部Ca+ +-depletion.D600和硝苯地平，这些组织的凝聚力的降低是一致的结果，在其他系统中，蛋白酶/Ca+ +-depletion失活Ca+ +依赖的细胞-细胞粘附机制。因此，我们认为，Ca+ +依赖性细胞间粘附在防止神经嵴细胞过早迁移中发挥了作用，并且这种粘附系统的定时失活通常有助于触发迁移的发生。Ca+ +通道阻断剂的结果表明，Ca+ +水平可能参与调节该系统。
SummaryTo investigate the control of the timing in the epithelio-mesenchymal transformation of the neural crest into a migrating population, neural anlagen (neural tube plus crest) were isolated from 2-day quail embryos by proteases in the presence of Ca+ + and explanted onto substrates favourable for neural crest cell migration. Explants isolated before normal migration had commenced required 3–8 h in vitro before neural crest cells started migration, but explants obtained at migratory stages showed an immediate onset of migration. The schedule was similar to that expected in vivo. When pre-migratory neural anlagen were isolated by protease in Ca+ +- and Mg+ +-free (CMF) medium, or when the protease was followed by a brief (5 min) exposure to CMF medium, neural crest cell migration commenced without delay, and the cohesion of the anlagen was impaired. Ca+ +-free medium duplicated the effects of CMF, but neither Mg+ +-free medium nor CMF treatment before treatment with protease stimulated migration and reduced cohesion. Precocious neural crest cell migration and reduced cohesion also followed when neural anlagen of pre-migratory stages were cultured with membrane. Ca+ +-channel antagonists D600 and Nifedipine, without any exernal Ca+ +-depletion.The decrease of cohesion of these tissues is consistent with results in other systems where protease/Ca+ +-depletion inactivates Ca+ +-dependent cell-cell adhesive mechanisms. Therefore, we suggest that Ca+ +-dependent cell-cell adhesions play a part in preventing neural crest cells from migrating precociously and that the timed inactivation of this adhesion system normally helps trigger the onset of migration. The results with blockers of Ca+ +-channels suggest that Ca+ + levels may be involved in regulating this system.
胚胎神经视网膜细胞中有两种不同的粘附机制。
影响因子：
2.7
作者：
Thomas,WA;Steinberg,MS
通讯作者：
Steinberg,MS
早期鸡胚胎发生中的细胞粘附分子。
DOI：
10.1073/pnas.79.21.6737
发表时间：
1982
影响因子：
11.1
作者：
Thiery,JP;Duband,JL;Rutishauser,U;Edelman,GM
通讯作者：
Edelman,GM