Sulfide production and oxidation by heterotrophic bacteria under aerobic conditions.
Sulfide production and oxidation by heterotrophic bacteria under aerobic conditions.
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有氧条件下异养细菌的硫化物产生和氧化
DOI：
10.1038/ismej.2017.125
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Xun L
中科院分区：
文献类型：
--
作者：
Xia Y;Lü C;Hou N;Xin Y;Liu J;Liu H;Xun L
异养细菌利用硫化物：醌氧化还原酶（SQR）和过硫双加氧酶（PDO）将硫化物（H2S， HS -和S2 -）氧化为亚硫酸盐和硫代硫酸盐，最近被报道为一种可能的高水平硫化物解毒机制。生物信息学分析显示，在测序的细菌基因组中，sqr和pdo基因是常见的，这意味着硫化物氧化可能具有其他生理功能。sqr以前被分为六种类型。在这里，我们将pdo分为三种类型，并表明一些异养细菌在有氧生长过程中产生并释放来自有机硫的H2S到顶空，而其他异养细菌，例如铜绿假单胞菌PAO1，具有sqr和pdo不释放H2S。当删除sqr和pdo基因时，突变体也释放H2S。从各种环境样品中均可分离出硫化物氧化性和非氧化性异养菌。在已发表的海洋宏基因组和亚转录组数据中，sqr和pdo基因也很常见，表明这些基因存在并表达。因此，异养细菌在有氧条件下生长在有机化合物上时，积极地产生和消耗硫化物。考虑到它们在地球上的丰富程度，它们对硫循环的贡献不容忽视。
Sulfide (H2S, HS− and S2−) oxidation to sulfite and thiosulfate by heterotrophic bacteria, using sulfide:quinone oxidoreductase (SQR) and persulfide dioxygenase (PDO), has recently been reported as a possible detoxification mechanism for sulfide at high levels. Bioinformatic analysis revealed that the sqr and pdo genes were common in sequenced bacterial genomes, implying the sulfide oxidation may have other physiological functions. SQRs have previously been classified into six types. Here we grouped PDOs into three types and showed that some heterotrophic bacteria produced and released H2S from organic sulfur into the headspace during aerobic growth, and others, for example, Pseudomonas aeruginosa PAO1, with sqr and pdo did not release H2S. When the sqr and pdo genes were deleted, the mutants also released H2S. Both sulfide-oxidizing and non-oxidizing heterotrophic bacteria were readily isolated from various environmental samples. The sqr and pdo genes were also common in the published marine metagenomic and metatranscriptomic data, indicating that the genes are present and expressed. Thus, heterotrophic bacteria actively produce and consume sulfide when growing on organic compounds under aerobic conditions. Given their abundance on Earth, their contribution to the sulfur cycle should not be overlooked.
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