Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance.
Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance.
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Tritipyrum 的转录组学和蛋白质组学综合分析为了解耐盐性的分子基础提供了见解
作者：
Yang R;Yang Z;Peng Z;He F;Shi L;Dong Y;Ren M;Zhang Q;Geng G;Zhang S
土壤盐分是制约作物生长和产量的主要环境胁迫。本文利用非数据获取蛋白质组学技术，研究了盐胁迫和恢复条件下雷公藤‘Y1805’的关键蛋白质及其生物学途径，以探讨其耐盐机制。在盐胁迫和恢复条件下，‘Y1805’共鉴定出44个差异表达蛋白和102个差异表达蛋白。蛋白质组-转录组联合分析表明，在盐胁迫和恢复条件下，分别有13和25个DEP的表达模式相同。‘Y1805’在两种条件下都出现了‘对刺激的反应’、‘抗氧化活性’、‘碳水化合物代谢’、‘氨基酸代谢’、‘信号转导’、‘运输和分解代谢’以及‘其他次生代谢产物的生物合成’。此外，与盐敏感小麦‘中国春’相比，‘能量代谢’和‘脂代谢’是恢复特有的途径，而在盐胁迫条件下的‘抗氧化活性’和‘分子功能调节’以及在恢复过程中的‘病毒粒子’和‘病毒粒子部分’是‘Y1805’特异的。‘Y1805’含有8个与盐胁迫反应相关的特异DEPS。‘Y1805’具有较强的耐盐性，其耐盐性可归因于细胞壁增强、活性氧清除、渗透调节、激素调节、短暂生长停滞、呼吸作用增强、转录调控和错误信息处理。这些数据将有助于理解耐盐性的分子机制，并有助于耐盐性小麦的育种。
Soil salinity is a major environmental stress that restricts crop growth and yield. Here, crucial proteins and biological pathways were investigated under salt-stress and recovery conditions in Tritipyrum ‘Y1805’ using the data-independent acquisition proteomics techniques to explore its salt-tolerance mechanism. In total, 44 and 102 differentially expressed proteins (DEPs) were identified in ‘Y1805’ under salt-stress and recovery conditions, respectively. A proteome-transcriptome-associated analysis revealed that the expression patterns of 13 and 25 DEPs were the same under salt-stress and recovery conditions, respectively. ‘Response to stimulus’, ‘antioxidant activity’, ‘carbohydrate metabolism’, ‘amino acid metabolism’, ‘signal transduction’, ‘transport and catabolism’ and ‘biosynthesis of other secondary metabolites’ were present under both conditions in ‘Y1805’. In addition, ‘energy metabolism’ and ‘lipid metabolism’ were recovery-specific pathways, while ‘antioxidant activity’, and ‘molecular function regulator’ under salt-stress conditions, and ‘virion’ and ‘virion part’ during recovery, were ‘Y1805’-specific compared with the salt-sensitive wheat ‘Chinese Spring’. ‘Y1805’ contained eight specific DEPs related to salt-stress responses. The strong salt tolerance of ‘Y1805’ could be attributed to the strengthened cell walls, reactive oxygen species scavenging, osmoregulation, phytohormone regulation, transient growth arrest, enhanced respiration, transcriptional regulation and error information processing. These data will facilitate an understanding of the molecular mechanisms of salt tolerance and aid in the breeding of salt-tolerant wheat.
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影响因子：
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作者：
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通讯作者：
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