Molecular cloning, characterization and expression modulation of four ferritins in black carp Mylopharyngodon piceus in response to Aeromonas hydrophila challenge
Molecular cloning, characterization and expression modulation of four ferritins in black carp Mylopharyngodon piceus in response to Aeromonas hydrophila challenge
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青鱼青鱼中四种铁蛋白响应嗜水气单胞菌挑战的分子克隆、表征和表达调节
DOI：
10.1016/j.aqrep.2019.100238
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发表时间：
2020-03
影响因子：
3.7
通讯作者：
Ye Jinyun
中科院分区：
文献类型：
--
作者：
Chen Shujian;Wu Chenglong;Xie Yuanyuan;Wu Yuancai;Dai Shurong;Wang Xiaowen;Li Ronghua;Ye Jinyun
铁蛋白（ferritin，FT）作为一种普遍存在的保守蛋白质，在免疫系统和铁储存系统中起着重要作用.利用同源克隆和cDNA末端快速扩增技术，从青鱼中克隆了4个铁蛋白（MpFT）的cDNA。MpFT-H1α、MpFT-H1 β、MpFT-M和MpFT-L分别含有177、177、176和173个氨基酸。BLAST分析表明，MpFTs与斑马鱼、虹鳟和人类等已知的FTs具有高度的同源性，在MpFT-H1α和MpFT-M的5 '-非翻译区（UTR）分别预测了一个典型的铁响应元件（IRE）。RT-PCR结果显示，青鱼血液、肝脏和心脏中MpFT 1a的相对表达量较高（P<0. 05）。MpFT 1b在青鱼肝脏和心脏中的表达量显著高于对照组（P< 0.05）。肝脏、血液和脑组织中MpFTL的转录水平相对较高（P< 0.05）。RT-PCR结果显示，青鱼肝脏和肠道中MpFTM的表达量显著高于肌肉中的表达量（P< 0.05）。感染后青鱼血液中FT-M和FT-L的表达变化比FT-H1 a和FT-H1b的表达变化更敏感。同样，在青鱼肝脏中，四种FTs的mRNA表达也有不同程度的上调，并在感染后12 h、12 h、6 h和48 h达到高峰。在肠道中，所有四种FT的转录变化在感染后24 h达到最高水平。这些结果表明，4种MpFTs可能在A.在这项研究中，
As ubiquitous and conserved proteins, ferritin (FT) plays important roles in immune system and iron storage system. Four cDNAs encoding ferritin (MpFT) were cloned from black carpMylopharyngodon piceususing homology cloning and rapid amplification of cDNA ends techniques. There were 177, 177, 176 and 173 amino acids in the MpFT-H1α, MpFT-H1β, MpFT-M and MpFT-L, respectively. BLAST analysis reveals that MpFTs shares high identities with other known FTs from zebrafish, Rainbow trout and Human, etc. A typical iron-responsive element (IRE) was predicted in the 5’-untranslated region (UTR) of MpFT-H1α and MpFT-M, respectively. The relative expression levels of MpFT1a were higher in the blood, liver and heart of black carp according to RT-PCR assays (P< 0.05). Higher expression levels of MpFT1b were shown in the liver and heart of black carp (P< 0.05). Relative higher transcriptional levels of MpFTL could be observed in the liver, blood and brain (P< 0.05). And higher expression levels of MpFTM were shown in the liver and intestine compared with these of in the muscle of black carp according to RT-PCR assays (P< 0.05). And the expression variations of FT-M and FT-L were more sensitive than these of FT-H1a and FT-H1b in the blood of black carp post infection. Similarly, the mRNA expression profiles of four FTs could also be differently upregulated and reached to the peak expression levels at 12h, 12h, 6h and 48h afterAeromonas hydrophilainfection in the liver of black carp. In the intestine, all the transcription variations of four FTs were reached to highest levels at 24 h post infection in this study. As a whole, these results indicated that four MpFTs might play important roles in innate immune defense duringA. hydrophilainfection in this study.
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影响因子：
4.7
作者：
C. Ren;Ting Chen;Xiao Jiang;Yanhong Wang;Chao-qun Hu
通讯作者：
C. Ren;Ting Chen;Xiao Jiang;Yanhong Wang;Chao-qun Hu
影响因子：
2.9
作者：
C. Jin;Chenghua Li;X. Su;Taiwu Li
通讯作者：
C. Jin;Chenghua Li;X. Su;Taiwu Li
DOI：
--
发表时间：
1995-06
期刊：
Molecular marine biology and biotechnology
影响因子：
--
作者：
O. Andersen;A. Dehli;H. Standal;T. A. Giskegjerde;R. Karstensen;K. Rorvik
通讯作者：
O. Andersen;A. Dehli;H. Standal;T. A. Giskegjerde;R. Karstensen;K. Rorvik
影响因子：
4.7
作者：
Chenglong Wu;Jinyun Ye;Jun’e Gao;Lian Chen;Zhibin Lu
通讯作者：
Chenglong Wu;Jinyun Ye;Jun’e Gao;Lian Chen;Zhibin Lu
影响因子：
2.9
作者：
Don Anushka Sandaruwan Elvitigala;Thanthrige Thiunuwan Priyathilaka;B. Lim;I. Whang;Sang-Yeob Yeo;C. Choi-
通讯作者：
Don Anushka Sandaruwan Elvitigala;Thanthrige Thiunuwan Priyathilaka;B. Lim;I. Whang;Sang-Yeob Yeo;C. Choi-