The anguibactin biosynthesis and transport genes are encoded in the chromosome of Vibrio harveyi: a possible evolutionary origin for the pJM1 plasmid-encoded system of Vibrio anguillarum?
The anguibactin biosynthesis and transport genes are encoded in the chromosome of Vibrio harveyi: a possible evolutionary origin for the pJM1 plasmid-encoded system of Vibrio anguillarum?
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DOI：
10.1002/mbo3.65
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发表时间：
2013-02
期刊：
影响因子：
3.4
通讯作者：
Crosa, Jorge H.
中科院分区：
文献类型：
--
作者：
Naka, Hiroaki;Actis, Luis A.;Crosa, Jorge H.
许多鳗弧菌血清型O1菌株携带65kb的pjm1型质粒，其中含有参与鳗丝素铁载体生物合成和运输的基因。anguibactin系统是鳗弧菌在限铁条件下生存的重要因子，是鳗弧菌非常重要的毒力因子。我们对基因组数据进行了比较分析，在哈维弧菌的染色体中发现了一簇含有anguibactin生物合成和转运基因的同源物。通过质谱和特异性生物分析，我们纯化了假定的鳗丝素铁载体，并证明它确实是鳗丝素。此外，我们通过诱变分析确定了该染色体簇中的两个基因，angR和fatA，它们分别参与了anguibactin的生物合成和运输。此外，我们发现V. harveyi FatA蛋白位于外膜部分，正如之前在V. anguillarum中所证明的那样。根据我们的数据，我们提出鳗鳗pJM1质粒中的鳗丝素生物合成和转运簇可能是从鳗丝素的染色体簇进化而来的，反之亦然。
Many Vibrio anguillarum serotype O1 strains carry 65-kb pJM1-type plasmids harboring genes involved in siderophore anguibactin biosynthesis and transport. The anguibactin system is an essential factor for V. anguillarum to survive under iron-limiting conditions, and as a consequence, it is a very important virulence factor of this bacterium. Our comparative analysis of genomic data identified a cluster harboring homologs of anguibactin biosynthesis and transport genes in the chromosome of Vibrio harveyi. We have purified the putative anguibactin siderophore and demonstrated that it is indeed anguibactin by mass spectrometry and specific bioassays. Furthermore, we characterized two genes, angR and fatA, in this chromosome cluster that, respectively, participate in anguibactin biosynthesis and transport as determined by mutagenesis analysis. Furthermore, we found that the V. harveyi FatA protein is located in the outer membrane fractions as previously demonstrated in V. anguillarum. Based on our data, we propose that the anguibactin biosynthesis and transport cluster in the V. anguillarum pJM1 plasmid have likely evolved from the chromosome cluster of V. harveyi or vice versa.
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原核生物进化的基因组学和系统发生学视角
影响因子：
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作者：
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通讯作者：
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DOI：
10.1079/9781845935542.0570
发表时间：
2011-01-01
期刊：
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作者：
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