Endogenous proviruses of random-bred chickens and ring-necked pheasants: analysis with restriction endonucleases.
Endogenous proviruses of random-bred chickens and ring-necked pheasants: analysis with restriction endonucleases.
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随机饲养鸡和环颈雉鸡的内源原病毒：用限制性内切核酸酶进行分析。
DOI：
10.1016/0042-6822(81)90540-7
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发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Varmus,HE
中科院分区：
文献类型：
--
作者：
Hughes,SH;Vogt,PK;Bishop,JM;Varmus,HE
我们通过限制性内切酶消化和核酸杂交，检测了18只随机饲养的褐来航鸡和褐刻鸡群以及8只环颈雉鸡的DNA中与禽肉瘤病毒（ASV）DNA同源的序列。这两个物种都具有与 ASV 的复制基因（gag、pol 和env）和转化基因（src）相关的序列。这些序列在随机饲养的鸡中的分布让人想起近交白来航鸡群的情况。与gag、pol和env相关的序列似乎驻留在与内源性鸡病毒RAV-O的原病毒非常相似的结构中，并且与src相关的序列似乎是细胞基因（或多个基因）。随机饲养的鸡群中存在的内源原病毒的数量变化很大，并且随机饲养的鸟类基因组中存在的原病毒位置与白来航所描述的位置不同。环颈雉中的内源ASV相关序列属于相同的两类；与 ASV 复制基因相关的序列可能存在于原病毒中，而与 src 相关的序列则存在于细胞基因中。然而，通过限制性内切酶分析和杂交，内源野鸡病毒与鸡病毒明显不同。这些观察结果支持这样的假设：cellularsrc(c-src) 具有与内源性原病毒不同的进化历史，而内源性原病毒显然是由种系感染产生的。鸡和雉鸡的内源性病毒虽然明显相关，但似乎经历了显着的独立进化，这表明这些病毒实现跨物种边界成功种系感染的频率与物种内成功种系感染的率相比较低。
We have examined, by digestion with restriction endonucleases and nucleic acid hybridization, sequences homologous to avian sarcoma virus (ASV) DNA in DNA from 18 random-bred chickens of the brown leghorn and brown nick flocks and 8 ring-necked pheasants. Both species have sequences related to the replicative genes (gag, pol, andenv) and to the transforming gene (src) of ASV. The disposition of these sequences in random-bred chickens is reminiscent of the situation in inbred white leghorn flocks; the sequences related togag, pol, andenvappear to reside in structures which closely resemble proviruses of the endogenous chicken virus RAV-O, and thesrc-related sequences appear to be a cellular gene (or genes). The number of endogenous proviruses present in the random-bred flocks is highly variable, and there are proviruses present at positions in the genomes of the random-bred birds different from those described for white leghorns. The endogenous ASV-related sequences in ring-necked pheasants fall into the same two categories; sequences related to the replicative genes of ASV probably reside in proviruses, and thesrc-related sequences in a cellular gene (or genes). However, the endogenous pheasant viruses are clearly distinct from those of chickens both by analysis with restriction endonucleases and by hybridization. These observations support the hypothesis that cellularsrc(c-src) has had a separate evolutionary history from the endogenous proviruses, which apparently arise by germ line infections. The endogenous viruses of chickens and pheasants, while clearly related, appear to have undergone significant independent evolution, which suggests that the frequency with which these viruses achieve a successful germ line infection across species boundaries is low compared with the rate of successful germ line infections within a species.
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