The Target MicroRNAs and Potential Underlying Mechanisms of Yiqi-Bushen-Tiaozhi Recipe against-Non-Alcoholic Steatohepatitis.
The Target MicroRNAs and Potential Underlying Mechanisms of Yiqi-Bushen-Tiaozhi Recipe against-Non-Alcoholic Steatohepatitis.
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DOI：
10.3389/fphar.2020.529553
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发表时间：
2020
影响因子：
5.6
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Hong W;Li S;Cai Y;Zhang T;Yang Q;He B;Yu J;Chen Z
microRNA（miRNAs）已成为非酒精性脂肪性肝病/非酒精性脂肪性肝炎（NAFLD/NASH）的潜在治疗靶点。中医药在NAFLD/NASH的预防或治疗中发挥着重要作用。然而，TCM抗NASH的miRNA靶点仍然在很大程度上未知。本研究表明益气补肾调脂方能有效减轻饮食诱导的C57 BL/6小鼠NASH。为了鉴定YBT的miRNA靶点并了解潜在的潜在机制，我们使用miRNA和mRNA深度测序数据结合不稳定性途径分析（IPA）进行了网络药理学研究。筛选出Mmu-let-7a-5 p、mmu-let-7 b-5 p、mmu-let-7 g-3 p和mmu-miR-106 b-3 p作为YBT的主要靶点。我们的研究结果表明，YBT可能通过调节这些可能调节炎症/免疫和氧化应激的miRNAs的表达来减轻NASH。本研究为进一步研究益气养阴汤通过调控miRNAs抗NASH的作用机制提供了参考。
MicroRNAs (miRNAs) have emerged as potential therapeutic targets for non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH). Traditional Chineses Medicine (TCM) plays an important role in the prevention or treatment of NAFLD/NASH. However, miRNA targets of TCM against NASH still remain largely unknown. Here, we showed that Yiqi-Bushen-Tiaozhi (YBT) recipe effectively attenuated diet-induced NASH in C57BL/6 mice. To identify the miRNA targets of YBT and understand the potential underlying mechanisms, we performed network pharmacology using miRNA and mRNA deep sequencing data combined with Ingenuity Pathway Analysis (IPA). Mmu-let-7a-5p, mmu-let-7b-5p, mmu-let-7g-3p and mmu-miR-106b-3p were screened as the main targets of YBT. Our results suggested that YBT might alleviate NASH by regulating the expression of these miRNAs that potentially modulate inflammation/immunity and oxidative stress. This study provides useful information for guiding future studies on the mechanism of YBT against NASH by regulating miRNAs.
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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