Maf-dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar T3SS export.
Maf-dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar T3SS export.
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DOI：
10.1111/mmi.12549
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发表时间：
2014-04
影响因子：
3.6
通讯作者：
Shaw JG
中科院分区：
文献类型：
--
作者：
Parker JL;Lowry RC;Couto NA;Wright PC;Stafford GP;Shaw JG
细菌游动是通过鞭毛单体聚合后通过专用的鞭毛III型分泌系统组装的细丝的旋转介导的。一些细菌用唾液酸相关的糖装饰鞭毛蛋白，这是运动所必需的。洞穴气单胞菌是这一过程的模式生物，因为它含有一个基因简单的糖基化系统，并用假胺酸（Pse）修饰其鞭毛蛋白。鞭毛蛋白糖基化与出口之间的联系尚未完全确定。我们研究了糖基化在缺乏Maf1的菌株的输出和组装过程中的作用，Maf1是一种在糖基化途径的后期阶段参与Pse转移到鞭毛蛋白上的蛋白质。免疫印迹证实，糖基化不是鞭毛蛋白输出所必需的，但对于纤维组装是必不可少的，因为非糖基化的鞭毛蛋白仍然分泌。在体内，即使在没有假胺酸的情况下，Maf1也能直接与其鞭毛蛋白底物相互作用。鞭毛蛋白伴侣突变体（Flagellin chaperone mutant, flaJ）的鞭毛蛋白糖基化表明，在伴侣蛋白结合和蛋白分泌之前，鞭毛蛋白糖基化发生在细胞质中。与糖基化鞭毛蛋白结合的优先伴侣蛋白揭示了其关键作用，表明该系统已经进化到有利于分泌具有聚合能力的糖基化形式。
Bacterial swimming is mediated by rotation of a filament that is assembled via polymerization of flagellin monomers after secretion via a dedicated flagellar Type III secretion system. Several bacteria decorate their flagellin with sialic acid related sugars that is essential for motility. Aeromonas caviae is a model organism for this process as it contains a genetically simple glycosylation system and decorates its flagellin with pseudaminic acid (Pse). The link between flagellin glycosylation and export has yet to be fully determined. We examined the role of glycosylation in the export and assembly process in a strain lacking Maf1, a protein involved in the transfer of Pse onto flagellin at the later stages of the glycosylation pathway. Immunoblotting, established that glycosylation is not required for flagellin export but is essential for filament assembly since non-glycosylated flagellin is still secreted. Maf1 interacts directly with its flagellin substrate in vivo, even in the absence of pseudaminic acid. Flagellin glycosylation in a flagellin chaperone mutant (flaJ) indicated that glycosylation occurs in the cytoplasm before chaperone binding and protein secretion. Preferential chaperone binding to glycosylated flagellin revealed its crucial role, indicating that this system has evolved to favour secretion of the polymerization competent glycosylated form.
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影响因子：
3.1
作者：
Eaton, KA;Suerbaum, S;Krakowka, S
通讯作者：
Krakowka, S
影响因子：
2.8
作者：
Canals, Rocio;Vilches, Silvia;Tomas, Juan M.
通讯作者：
Tomas, Juan M.
影响因子：
6.7
作者：
Arnold R;Brandmaier S;Kleine F;Tischler P;Heinz E;Behrens S;Niinikoski A;Mewes HW;Horn M;Rattei T
通讯作者：
Rattei T
影响因子：
3.6
作者：
Blocker, A;Jouihri, N;Allaoui, A
通讯作者：
Allaoui, A
影响因子：
3.6
作者：
Guerry, P;Ewing, CP;Logan, S
通讯作者：
Logan, S