C-type lectins from the nematode parasites Heligmosomoides polygyrus and Nippostrongylus brasiliensis.
C-type lectins from the nematode parasites Heligmosomoides polygyrus and Nippostrongylus brasiliensis.
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DOI：
10.1016/j.parint.2009.08.011
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发表时间：
2009-12
影响因子：
1.9
通讯作者：
Maizels RM
中科院分区：
文献类型：
--
作者：
Harcus Y;Nicoll G;Murray J;Filbey K;Gomez-Escobar N;Maizels RM
c型凝集素超家族在迄今研究的所有后生动物门中都有很高的代表性。这个超家族的许多成员在对抗感染的先天免疫防御中很重要，而其他成员则在发育和结构中起关键作用。在超家族中，许多蛋白质含有多个典型的碳水化合物识别结构域（CRDs），以及额外的非凝集素结构域。本文研究了两种广泛应用于实验啮齿动物系统的胃肠道线虫寄生虫——多回Heligmosomoides polygyrus和巴西尼波圆线虫（Nippostrongylus brasiliensis）。从cDNA文库中分离到3个新的c型凝集素；它们都是具有短附加n端结构域的单crd蛋白。Hp-CTL-1蛋白含156个氨基酸，Nb-CTL-1蛋白含191个氨基酸，Nb-CTL-2蛋白含183个氨基酸；它们都编码预测的信号肽，以及CTL超家族特征的关键保守序列基序。这些凝集素与秀丽隐杆线虫的CLEC-48、49和50以及哺乳动物免疫系统蛋白CD23和CD206的凝集素结构域最为相似。RT-PCR结果显示，这些多回螺蛳和巴西巴西螺蛳基因主要在居住在肠道的成虫阶段表达，尽管Nb-CTL-2转录本在自由生活的感染性幼虫（L3）阶段也很突出。ELISA检测发现，Hp-CTL-1和Nb-CTL-1的多克隆抗体与两种蛋白均有反应，Western blot分析发现，巴西黑蚊（NES）成虫分泌蛋白中有一个15 kda的条带，多回黑蚊（HES）分泌蛋白中有一个19 kda的条带。抗ctl -1抗体也能与成虫多回毛囊角质层强结合。因此，活的寄生虫释放c型凝集素，这些凝集素与哺乳动物宿主免疫系统的一些关键受体同源，从而提高了这些产物以某种方式干扰免疫识别或效应功能的可能性。
The C-type lectin superfamily is highly represented in all metazoan phyla so far studied. Many members of this superfamily are important in innate immune defences against infection, while others serve key developmental and structural roles. Within the superfamily, many proteins contain multiple canonical carbohydrate-recognition domains (CRDs), together with additional non-lectin domains. In this report, we have studied two gastrointestinal nematode parasites which are widely used in experimental rodent systems, Heligmosomoides polygyrus and Nippostrongylus brasiliensis. From cDNA libraries, we have isolated 3 new C-type lectins from these species; all are single-CRD proteins with short additional N-terminal domains. The predicted Hp-CTL-1 protein contains 156 aa, Nb-CTL-1 191 aa and Nb-CTL-2 183 aa; all encode predicted signal peptides, as well as key conserved sequence motifs characteristic of the CTL superfamily. These lectins are most similar to C. elegans CLEC-48, 49 and 50, as well as to the lectin domains of mammalian immune system proteins CD23 and CD206. RT-PCR showed that these H. polygyrus and N. brasiliensis genes are primarily expressed in the gut-dwelling adult stages, although Nb-CTL-2 transcripts are also prominent in the free-living infective larval (L3) stage. Polyclonal antibodies raised to Hp-CTL-1 and Nb-CTL-1 reacted to both proteins by ELISA, and in Western blot analysis recognised a 15-kDa band in secreted proteins of adult N. brasiliensis (NES) and a 19-kDa band in H. polygyrus ES (HES). Anti-CTL-1 antibody also bound strongly to the cuticle of adult H. polygyrus. Hence, live parasites release C-type lectins homologous to some key receptors of the mammalian host immune system, raising the possibility that these products interfere in some manner with immunological recognition or effector function.
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影响因子：
64.5
作者：
Geijtenbeek, TBH;Torensma, R;Figdor, CG
通讯作者：
Figdor, CG
DOI：
10.1038/nri2569
发表时间：
2009-07
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Geijtenbeek TB;Gringhuis SI
通讯作者：
Gringhuis SI
影响因子：
5.4
作者：
Finney, Constance A M;Taylor, Matthew D;Wilson, Mark S;Maizels, Rick M
通讯作者：
Maizels, Rick M
影响因子：
2.8
作者：
Andrews, RK;Berndt, MC
通讯作者：
Berndt, MC
影响因子：
2.1
作者：
Lawrence, RA;Gray, CA;Maizels, RM
通讯作者：
Maizels, RM