A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells.

A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells.
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用于少量细胞的质谱流式细胞仪分析的载体策略。

DOI:
10.1007/978-1-0716-0266-9_2
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发表时间:
2020
期刊:
Methods Mol Biol
影响因子:
--
通讯作者:
Fu Guo
Fu Guo
中科院分区:
其他
文献类型:
--
作者:
Jia Xian;Zhou Xiaojuan;Zheng Haiping;Jiang Shan;Weng Jiannan;Huang Lei;Du Zhiqiang;Xiao Changchun;Zhang Lei;Chen Xiao Lei;Fu Guo

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The recent launch of mass cytometry or cytometry by time of flight (CyTOF) has revolutionized flow cytometry. Similar to fluorescence flow cytometry, a key challenge for CyTOF is to analyze samples of limited amount or very rare cell populations under various experimental settings. Here we describe a carrier strategy that significantly reduces the required sample amount without losing analytical resolution. We were able to detect as few as 5 × 104human peripheral blood mononuclear cells (PBMCs) using this method. This simple method thus enables the maximal usage of valuable clinical samples.
免疫反应中的异质性:从种群到单个细胞。
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