A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells.
A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells.
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用于少量细胞的质谱流式细胞仪分析的载体策略。
DOI:
10.1007/978-1-0716-0266-9_2
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发表时间:
2020
期刊:
影响因子:
--
通讯作者:
Fu Guo
中科院分区:
文献类型:
--
作者:
Jia Xian;Zhou Xiaojuan;Zheng Haiping;Jiang Shan;Weng Jiannan;Huang Lei;Du Zhiqiang;Xiao Changchun;Zhang Lei;Chen Xiao Lei;Fu Guo
The recent launch of mass cytometry or cytometry by time of flight (CyTOF) has revolutionized flow cytometry. Similar to fluorescence flow cytometry, a key challenge for CyTOF is to analyze samples of limited amount or very rare cell populations under various experimental settings. Here we describe a carrier strategy that significantly reduces the required sample amount without losing analytical resolution. We were able to detect as few as 5 × 104human peripheral blood mononuclear cells (PBMCs) using this method. This simple method thus enables the maximal usage of valuable clinical samples.
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影响因子:
16.8
作者:
Satija R;Shalek AK
通讯作者:
Shalek AK
DOI:
10.1007/978-1-4939-2963-4_7
发表时间:
2015
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
作者:
Leipold MD;Newell EW;Maecker HT
通讯作者:
Maecker HT
DOI:
10.1126/science.1198704
发表时间:
2011-05-06
期刊:
Science (New York, N.Y.)
影响因子:
--
作者:
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者:
Nolan GP
影响因子:
46.9
作者:
Good, Zinaida;Borges, Luciene;Bendall, Sean C.
通讯作者:
Bendall, Sean C.
影响因子:
6
作者:
L. Maaten;Geoffrey E. Hinton
通讯作者:
L. Maaten;Geoffrey E. Hinton