Prenatal and Lactational Exposure to Bisphenol A in Mice Alters Expression of Genes Involved in Cortical Barrel Development without Morphological Changes.
Prenatal and Lactational Exposure to Bisphenol A in Mice Alters Expression of Genes Involved in Cortical Barrel Development without Morphological Changes.
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DOI：
10.1267/ahc.10042
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发表时间：
2011-02-26
影响因子：
2.4
通讯作者：
Fushiki S
中科院分区：
文献类型：
--
作者：
Han L;Itoh K;Yaoi T;Moriwaki S;Kato S;Nakamura K;Fushiki S
据报道，新生儿重症监护病房的早产儿暴露于高比率的双酚a (BPA)，一种内分泌干扰化学物质。我们之前的研究表明，皮质丘脑投影被产前暴露于BPA破坏，即使在成年小鼠中也持续存在。因此，我们分析了产前和哺乳期暴露于低剂量双酚a是否会影响皮质桶状体、丘脑桶状体和脑干桶状体的形成，并从组织学和桶状体发育相关基因的表达方面进行了分析。从胚胎第0天（E0）至出生后3周（P3W），每天皮下注射20µg/kg BPA，对照组小鼠单独注射。采用细胞色素C氧化酶（COX）染色法检测成年小鼠的桶状、桶状和桶状。bpa暴露组与对照组在成年小鼠脑总面积和间隔面积以及后内侧桶亚野（PMBSF）、桶状体和桶状体的图案上均无显著差异。在出生后第1天（PD1）、PD4和PD8的发育研究显示，两组的皮质桶在PD4时隐约出现，在PD8时完全形成。一些基因的表达模式随性别和处理而发生时空变化。这些结果表明，在产前和哺乳期暴露于低剂量双酚a后，三叉神经投射和丘脑到皮质桶的传递得以保留，尽管产前暴露于双酚a已被证明会破坏皮质丘脑投射。
It has been reported that premature infants in neonatal intensive care units are exposed to a high rate of bisphenol A (BPA), an endocrine disrupting chemical. Our previous studies demonstrated that corticothalamic projection was disrupted by prenatal exposure to BPA, which persisted even in adult mice. We therefore analyzed whether prenatal and lactational exposure to low doses of BPA affected the formation of the cortical barrel, the barreloid of the thalamus, and the barrelette of the brainstem in terms of the histology and the expression of genes involved in the barrel development. Pregnant mice were injected subcutaneously with 20 µg/kg of BPA daily from embryonic day 0 (E0) to postnatal 3 weeks (P3W), while the control mice received a vehicle alone. The barrel, barreloid and barrelette of the adult mice were examined by cytochrome C oxidase (COX) staining. There were no significant differences in the total and septal areas and the patterning of the posterior medial barrel subfield (PMBSF), barreloid and barrelette, between the BPA-exposure and control groups in the adult mice. The developmental study at postnatal day 1 (PD1), PD4 and PD8 revealed that the cortical barrel vaguely appeared at PD4 and completely formed at PD8 in both groups. The expression pattern of some genes was spatiotemporally altered depending on the sex and the treatment. These results suggest that the trigeminal projection and the thalamic relay to the cortical barrel were spared after prenatal and lactational exposure to low doses of BPA, although prenatal exposure to BPA was previously shown to disrupt the corticothalamic projection.
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DOI：
10.1016/j.ijdevneu.2005.04.003
发表时间：
2005-10-01
影响因子：
1.8
作者：
Lush, ME;Ma, L;Parada, LF
通讯作者：
Parada, LF
新生儿重症监护室早产儿接触双酚A和其他酚类物质。
影响因子：
10.4
作者：
Calafat, Antonia M.;Weuve, Jennifer;Ye, Xiaoyun;Jia, Lily T.;Hu, Howard;Ringer, Steven;Huttner, Ken;Hauser, Russ
通讯作者：
Hauser, Russ
影响因子：
2.5
作者：
DURHAM, D;WOOLSEY, TA
通讯作者：
WOOLSEY, TA
影响因子：
5.3
作者：
Gheorghita, Fulvia;Kraftsik, Rudolf;Welker, Egbert
通讯作者：
Welker, Egbert
DOI：
10.1016/s0165-3806(03)00012-9
发表时间：
2003-04-14
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Muneoka, KT;Borella, A;Whitaker-Azmitia, PM
通讯作者：
Whitaker-Azmitia, PM