Ex vivo nanofiber expansion and genetic modification of human cord blood-derived progenitor/stem cells enhances vasculogenesis.
Ex vivo nanofiber expansion and genetic modification of human cord blood-derived progenitor/stem cells enhances vasculogenesis.
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DOI：
10.3727/096368909788534870
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发表时间：
2009
影响因子：
3.3
通讯作者：
Pompili VJ
中科院分区：
文献类型：
--
作者：
Das H;Abdulhameed N;Joseph M;Sakthivel R;Mao HQ;Pompili VJ
干细胞疗法在治疗缺血性疾病方面前景看好；然而，干细胞在老年和疾病患者中的有限可获得性和质量降低限制了其治疗应用。在这里，我们报道了一种基于纳米纤维的干细胞体外扩增技术，并通过过表达人脐血祖细胞来增强治疗性干细胞的血管生成潜力。在基于纳米纤维的无血清体外扩增技术中，祖细胞扩增了~225倍，没有诱导分化。扩增的细胞表达高水平的干细胞归巢受体CXCR4和黏附分子LFA-1。纳米纤维扩增的干细胞有效地摄取AcLDL，并在体外移行试验中高效迁移。这些扩增的细胞在体外也可以分化为内皮细胞和平滑肌细胞。在NOD/SCID小鼠后肢血管损伤模型中，纳米纤维扩张的细胞在血流恢复方面更有效，并且VEGF164和PDGF-BB的过度表达进一步增强了这种作用。数据表明，基于纳米纤维的体外扩增技术可以提供必要数量的治疗性干细胞。此外，前血管生成因子在祖细胞中的过度表达可能会改善自体或同种异体干细胞治疗缺血性疾病。
The stem cell therapy for treating ischemic diseases is promising; however, the limited availability and compromised quality of progenitor cells in aged and diseased patients limit its therapeutic use. Here we report a nanofiber-based ex vivo stem cell expansion technology and proangiogenic growth factors overexpression of human umbilical cord blood (UCB)-derived progenitor cells to enhance angiogenic potential of therapeutic stem cells. The progenitor cells were expanded ~225-fold on nanofiber-based serum-free ex vivo expansion culture technique without inducing differentiation. The expanded cells express high levels of stem cell homing receptor, CXCR4, and adhesion molecule, LFA-1. The nanofiber-expanded stem cells uptake AcLDL effectively, and migrate efficiently in an in vitro transmigration assay. These expanded cells can also differentiate into endothelial and smooth muscle cells in vitro. In a NOD/SCID mouse hind limb vascular injury model, nanofiber-expanded cells were more effective in blood flow restoration and this effect was further augmented by VEGF164 and PDGF-BB, growth factor overexpression. The data indicate that nanofiber-based ex vivo expansion technology can provide an essential number of therapeutic stem cells. Additionally, proangiogenic growth factors overexpression in progenitor cells can potentially improve autologous or allogeneic stem cell therapy for ischemic diseases.
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影响因子：
64.8
作者：
Murry, CE;Soonpaa, MH;Field, LJ
通讯作者：
Field, LJ
DOI：
10.1196/annals.1402.086
发表时间：
2007-01-01
期刊：
SKELETAL BIOLOGY AND MEDICINE, PT A
影响因子：
--
作者：
Mendez-Ferrer, Simon;Frenette, Paul S.
通讯作者：
Frenette, Paul S.
影响因子：
20.3
作者：
Salvucci, O;Yao, L;Tosato, G
通讯作者：
Tosato, G
影响因子：
4
作者：
Kano, MR;Morishita, Y;Miyazawa, K
通讯作者：
Miyazawa, K
影响因子：
14
作者：
Chua, Kian-Ngiap;Chai, Chou;Mao, Hai-Quan
通讯作者：
Mao, Hai-Quan