Zebrafish chordin-like and chordin are functionally redundant in regulating patterning of the dorsoventral axis.
Zebrafish chordin-like and chordin are functionally redundant in regulating patterning of the dorsoventral axis.
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DOI：
10.1016/j.ydbio.2010.03.001
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发表时间：
2010-05-15
影响因子：
2.7
通讯作者：
Greenspan DS
中科院分区：
文献类型：
--
作者：
Branam AM;Hoffman GG;Pelegri F;Greenspan DS
Chordin是一组富含半胱氨酸结构域的蛋白质的原型，其结合并调节各种TGFβ样配体的信号传导。Chordin-like 1和Chordin-like 2（CHL 1和CHL 2）是这一组的两个成员，已经在人、小鼠和鸡中描述。然而，由于缺乏功能丧失分析，CHL 1和2在早期发育中的体内作用尚不清楚。在这里，我们确定和表征斑马鱼，斑马鱼，CHL（叶绿素）。chl基因位于染色体21的区域上，该区域与携带CHL 2基因的鼠染色体7的区域同线。无法确定一个单独的斑马鱼基因对应的哺乳动物CHL 1基因表明，叶绿素可能在斑马鱼中的作用，分布在其他物种的CHL 1和CHL 2之间。叶绿素是一个母亲的因素，也是合子在发育后期表达，并具有时空表达模式不同，但重叠的斑马鱼脊索蛋白（Chd），这表明差异，但也可能重叠的两种蛋白质的发育作用。Chl与Chd一样，在过度表达时使胚胎背侧化，并被BMP 1切割，BMP 1拮抗这种活性。丧失功能的实验表明，叶绿素作为BMP拮抗剂的功能重叠，是多余的，与Chd在形成背腹轴。
Chordin is the prototype of a group of cysteine-rich domain-containing proteins that bind and modulate signaling of various TGFβ-like ligands. Chordin-like 1 and 2 (CHL1 and 2) are two members of this group that have been described in human, mouse and chick. However, in vivo roles for CHL1 and 2 in early development are unknown, due to lack of loss-of-function analysis. Here we identify and characterize zebrafish, Danio rerio, CHL (Chl). The chl gene is on a region of chromosome 21 syntenic with the area of murine chromosome 7 bearing the CHL2 gene. Inability to identify a separate zebrafish gene corresponding to the mammalian CHL1 gene suggests that Chl may serve roles in zebrafish distributed between CHL1 and CHL2 in other species. Chl is a maternal factor that is also zygotically expressed later in development, and has spatiotemporal expression patterns that differ from, but overlap those of zebrafish chordin (Chd), suggesting differences, but also possible overlap in developmental roles of the two proteins. Chl, like Chd, dorsalizes embryos upon overexpression, and is cleaved by BMP1, which antagonizes this activity. Loss-of-function experiments demonstrate that Chl serves as a BMP antagonist with functions that overlap and are redundant with those of Chd in forming the dorsoventral axis.
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影响因子：
5.3
作者：
Pappano, WN;Steiglitz, BM;Greenspan, DS
通讯作者：
Greenspan, DS
影响因子：
1.5
作者：
Draper, BW;Morcos, PA;Kimmel, CB
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Kimmel, CB
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21.3
作者：
Muraoka, O;Shimizu, T;Hibi, M
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Hibi, M
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作者：
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通讯作者：
Strahle, U
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64.5
作者：
Marques, G;Musacchio, M;OConnor, MB
通讯作者：
OConnor, MB