Chronic spinal hemisection in rats induces a progressive decline in transmission in uninjured fibers to motoneurons.
Chronic spinal hemisection in rats induces a progressive decline in transmission in uninjured fibers to motoneurons.
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DOI：
10.1016/j.expneurol.2009.01.004
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发表时间：
2009-04
影响因子：
5.3
通讯作者：
Mendell, Lorne M.
中科院分区：
文献类型：
--
作者：
Arvanian, Victor L.;Schnell, Lisa;Lou, Li;Golshani, Roozbeh;Hunanyan, Arsen;Ghosh, Arko;Pearse, Damien D.;Robinson, John K.;Schwab, Martin E.;Fawcett, James W.;Mendell, Lorne M.
关键词：
虽然大多数脊髓损伤在解剖学上是不完全的，但在部分损伤的人和大鼠中仅观察到有限的功能恢复。为了解决为什么幸存的纤维不能介导更完全的恢复，我们评估了生理和解剖状态的备用纤维单侧半切（HX）后的成年大鼠胸脊髓。我们在L5（HX下方）对HX上方（T6）和下方（L1）的对侧白色物质进行了细胞内和细胞外记录。从HX后1-2周开始，T6的反应显示HX对面纤维的振幅降低，潜伏期增加和刺激阈值升高。超微结构分析显示，脱髓鞘的完整轴突对侧的HX，与时间过程类似的传导变化。行为学研究表明，部分恢复，当传导缺陷开始逮捕。总之，这项研究是第一次证明的延迟下降的传输通过幸存的轴突个别腰运动神经元在慢性阶段的不完全性脊髓损伤的成年大鼠。这些结果表明，完整的纤维中存在慢性病理状态，需要及时治疗以尽量减少这种情况。
Although most spinal cord injuries are anatomically incomplete, only limited functional recovery has been observed in people and rats with partial lesions. To address why surviving fibers cannot mediate more complete recovery, we evaluated the physiological and anatomical status of spared fibers after unilateral hemisection (HX) of thoracic spinal cord in adult rats. We made intracellular and extracellular recordings at L5 (below HX) in response to electrical stimulation of contralateral white matter above (T6) and below (L1) HX. Responses from T6 displayed reduced amplitude, increased latency and elevated stimulus threshold in the fibers across from HX, beginning 1–2 weeks after HX. Ultrastructural analysis revealed demyelination of intact axons contralateral to the HX, with a time course similar to the conduction changes. Behavioral studies indicated partial recovery which arrested when conduction deficits began. In conclusion, this study is the first demonstration of the delayed decline of transmission through surviving axons to individual lumbar motoneurons during chronic stage of incomplete spinal cord injury in adult rats. These findings suggest a chronic pathological state in intact fibers and necessity for prompt treatment to minimize it.
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影响因子：
5.3
作者：
Arvanian, VL;Bowers, WJ;Mendell, LM
通讯作者：
Mendell, LM
影响因子：
82.9
作者：
Courtine, Gregoire;Song, Bingbing;Sofroniew, Michael V.
通讯作者：
Sofroniew, Michael V.
影响因子：
2.5
作者：
Antonino-Green, DM;Cheng, JG;Magnuson, DSK
通讯作者：
Magnuson, DSK
影响因子：
3.1
作者：
Gramsbergen, Albert
通讯作者：
Gramsbergen, Albert
影响因子：
5.3
作者：
Guest, JD;Hiester, ED;Bunge, RP
通讯作者：
Bunge, RP