Ozone augments interleukin-8 production induced by ambient particulate matter.
Ozone augments interleukin-8 production induced by ambient particulate matter.
复制标题
DOI：
10.1186/s41021-018-0102-7
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Watanabe M
中科院分区：
文献类型：
--
作者：
Kurai J;Onuma K;Sano H;Okada F;Watanabe M
实验性和受控的人体接触研究表明，环境颗粒物和臭氧对健康的叠加效应。一些流行病学研究表明，环境颗粒物成分对环境颗粒物和臭氧对健康的综合影响很重要。然而，很少有研究调查臭氧是否会改变环境颗粒物对促炎细胞因子产生的影响。在这项研究中，评估了臭氧对促炎细胞因子产生的影响，以响应环境颗粒物。收集环境中小于1 μm的悬浮颗粒物，通过0.12 ppm和0.24 ppm的臭氧使悬浮颗粒物冒泡。1 μg/mL含臭氧和不含臭氧气泡颗粒物的溶液刺激THP1细胞。上层清液的浓度interleukin-8 THP1细胞刺激收集颗粒物溶解在溶液中分别为108.3±24.7 pg / mL没有臭氧接触,165.0±26.1 pg / mL 0.12 ppm臭氧冒泡1分钟,175.1±33.1 pg / mL 0.12 ppm为5分钟,183.3±17.8 pg / mL 0.12 ppm,持续15分钟,167.8±35.9 pg / mL 0.24 ppm为1分钟,209.2±8.4 pg / mL 0.24 ppm为5分钟,和209.3±14.3 pg / mL为15分钟0.24 ppm。臭氧显著增加了白细胞介素-8浓度，与未接触臭氧和仅接触溶剂的颗粒物相比（8.2±0.9 pg/mL），臭氧浓度依赖于臭氧浓度。在有或没有臭氧泡的溶液中收集的颗粒物对白细胞介素-6的产生没有影响。抗氧化剂n-乙酰- l-半胱氨酸显著抑制颗粒物质溶液诱导的白细胞介素-8的增加，与臭氧暴露无关。收集颗粒物溶液中的活性氧浓度与臭氧鼓泡无关。臭氧可能通过与活性氧无关的机制增加白细胞介素-8的产生，以响应环境颗粒物。这些结果支持了关于环境颗粒物和臭氧对人类健康综合影响的流行病学证据。
Experimental and controlled human exposure studies have demonstrated additive effects of ambient particulate matter and ozone on health. A few epidemiological studies have suggested that ambient particulate matter components are important for the combined effects of ambient particulate matter and ozone on health. However, few studies have examined whether ozone changes the effects of ambient particulate matter on pro-inflammatory cytokine production. In this study, the influence of ozone on pro-inflammatory cytokine production in response to ambient particulate matter was evaluated. Ambient particulate matter smaller than 1 μm was collected and the suspension of this particulate matter was bubbled through 0.12 ppm and 0.24 ppm ozone. THP1 cells were stimulated by the solution containing the particulate matter with and without bubbling through ozone at 1 μg/mL. The interleukin-8 concentrations in the supernatants of THP1 cells stimulated by collected particulate matter dissolved in solution were 108.3 ± 24.7 pg/mL without ozone exposure, 165.0 ± 26.1 pg/mL for 0.12 ppm ozone bubbling for 1 min, 175.1 ± 33.1 pg/mL for 0.12 ppm for 5 min, 183.3 ± 17.8 pg/mL for 0.12 ppm for 15 min, 167.8 ± 35.9 pg/mL for 0.24 ppm for 1 min, 209.2 ± 8.4 pg/mL for 0.24 ppm for 5 min, and 209.3 ± 14.3 pg/mL for 0.24 ppm for 15 min. Ozone significantly increased interleukin-8 concentrations compared to those for particulate matter dissolved in solution without ozone exposure and the solvent only (8.2 ± 0.9 pg/mL) in an ozone concentration-dependent manner. Collected particulate matter in solutions with or without bubbling through ozone had no effect on interleukin-6 production. The antioxidant N-acetyl-L-cysteine significantly inhibited the increases in interleukin-8 induced by solutions with particulate matter, regardless of ozone exposure. The reactive oxygen species concentration in solutions with collected particulate matter was not associated with ozone bubbling. Ozone may augment the production of interleukin-8 in response to ambient particulate matter by a mechanism unrelated to reactive oxygen species. These results support the epidemiological evidence for combined effects of ambient particulate matter and ozone on human health.
登录
查看更多内容
影响因子：
10
作者：
Atkinson RW;Kang S;Anderson HR;Mills IC;Walton HA
通讯作者：
Walton HA
影响因子：
2.9
作者：
Atkinson RW;Butland BK;Dimitroulopoulou C;Heal MR;Stedman JR;Carslaw N;Jarvis D;Heaviside C;Vardoulakis S;Walton H;Anderson HR
通讯作者：
Anderson HR
影响因子：
10.4
作者：
Gurgueira SA;Lawrence J;Coull B;Murthy GG;González-Flecha B
通讯作者：
González-Flecha B
在多站点时间序列研究中，在臭氧与死亡率之间的短期关联上，颗粒物的潜在混淆。
影响因子：
10.4
作者：
Bell, Michelle L.;Kim, Jee Young;Dominici, Francesca
通讯作者：
Dominici, Francesca
影响因子：
3.3
作者：
Kurt OK;Zhang J;Pinkerton KE
通讯作者：
Pinkerton KE