Dynamic QTL analysis and candidate gene mapping for waterlogging tolerance at maize seedling stage.
Dynamic QTL analysis and candidate gene mapping for waterlogging tolerance at maize seedling stage.
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玉米苗期耐涝性动态QTL分析和候选基因定位。
DOI：
10.1371/journal.pone.0079305
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Qiu F
中科院分区：
文献类型：
--
作者：
Osman KA;Tang B;Wang Y;Chen J;Yu F;Li L;Han X;Zhang Z;Yan J;Zheng Y;Yue B;Qiu F
土壤渍水是影响玉米生长和产量的主要非生物胁迫之一。利用混合线性模型和包含式复合区间作图法，在淹水和对照条件下，通过条件分析确定了与株高、根长、根干重、地上部干重和总干重相关的基因或数量性状基因座(QTL)的动态表达。3d|0d、6d|3d和9d|6d分别检测到13个、19个和23个QTL。每个QTL的效应中等，分布在9条染色体上，单对表型变异的贡献率为4.14-18.88%。在苗木发育的两个持续阶段检测到6个QTL(ph6-1、RL1-2、sdw4-1、sdw7-1、tdw4-1和tdw7-1)，能够反映基因的连续表达，其余的QTL只在一个阶段检测到。因此，大多数QTL的表达受发育状况的影响。为了提供更多关于相应基因在耐涝中的作用的证据，对表达序列标签标记和microRNAs进行了定位。在第1、4、6、7和9号染色体上观察到7个候选基因与已鉴定的QTL共定位，可能是重要的耐涝候选基因。这些结果为理解QTL在不同逆境时期选择性表达的遗传基础和共定位性状的共同遗传控制机制提供了一个很好的起点。
Soil waterlogging is one of the major abiotic stresses adversely affecting maize growth and yield. To identify dynamic expression of genes or quantitative trait loci (QTL), QTL associated with plant height, root length, root dry weight, shoot dry weight and total dry weight were identified via conditional analysis in a mixed linear model and inclusive composite interval mapping method at three respective periods under waterlogging and control conditions. A total of 13, 19 and 23 QTL were detected at stages 3D|0D (the period during 0–3 d of waterlogging), 6D|3D and 9D|6D, respectively. The effects of each QTL were moderate and distributed over nine chromosomes, singly explaining 4.14–18.88% of the phenotypic variation. Six QTL (ph6-1, rl1-2, sdw4-1, sdw7-1, tdw4-1 and tdw7-1) were identified at two consistent stages of seedling development, which could reflect a continuous expression of genes; the remaining QTL were detected at only one stage. Thus, expression of most QTL was influenced by the developmental status. In order to provide additional evidence regarding the role of corresponding genes in waterlogging tolerance, mapping of Expressed Sequence Tags markers and microRNAs were conducted. Seven candidate genes were observed to co-localize with the identified QTL on chromosomes 1, 4, 6, 7 and 9, and may be important candidate genes for waterlogging tolerance. These results are a good starting point for understanding the genetic basis for selectively expressing of QTL in different stress periods and the common genetic control mechanism of the co-localized traits.
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