Evidence that Maackia amurensis seed lectin (MASL) exerts pleiotropic actions on oral squamous cells with potential to inhibit SARS-CoV-2 infection and COVID-19 disease progression.
Evidence that Maackia amurensis seed lectin (MASL) exerts pleiotropic actions on oral squamous cells with potential to inhibit SARS-CoV-2 infection and COVID-19 disease progression.
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马兰籽凝集素（MASL）对口腔鳞状细胞发挥多效作用，可能抑制SARS-CoV-2感染和COVID-19疾病进展的证据
DOI：
10.1016/j.yexcr.2021.112594
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发表时间：
2021-06-01
影响因子：
3.7
通讯作者：
Goldberg GS
中科院分区：
文献类型：
--
作者：
Sheehan SA;Hamilton KL;Retzbach EP;Balachandran P;Krishnan H;Leone P;Lopez-Gonzalez M;Suryavanshi S;Kumar P;Russo R;Goldberg GS
COVID-19 于 1 月被宣布为国际公共卫生紧急事件，并于 2020 年 3 月被宣布为大流行。截至 2021 年 3 月，全球已有超过 1.25 亿例确诊的 COVID-19 病例，已导致超过 270 万人死亡。COVID-19 是由 SARS-CoV-2 病毒引起的。 SARS-CoV-2 呈现出一种表面“刺突”蛋白，可与 ACE2 受体结合以感染宿主细胞。除了呼吸道外，SARS-Cov-2 还可以感染口腔粘膜细胞，这些细胞也表达 ACE2 受体。刺突蛋白和 ACE2 蛋白经过唾液酸修饰高度糖基化，可指导病毒与宿主的相互作用和感染。山槐种子凝集素 (MASL) 对唾液酸修饰蛋白具有很强的亲和力，可用作抗病毒剂。在此，我们报告 MASL 以 ACE2 受体为靶点，降低 ACE2 表达和糖基化，抑制 SARS-CoV-2 刺突蛋白的结合，并降低口腔上皮细胞引起 COVID-19 患者 ARDS 的炎症介质的表达。此外，我们报告 MASL 还可以抑制培养物中肾上皮细胞的 SARS-CoV-2 感染。这项工作确定 MASL 是一种具有抑制 SARS-CoV-2 感染和 COVID-19 相关炎症综合征潜力的药物。
COVID-19 was declared an international public health emergency in January, and a pandemic in March of 2020. There are over 125 million confirmed COVID-19 cases that have caused over 2.7 million deaths worldwide as of March 2021. COVID-19 is caused by the SARS-CoV-2 virus. SARS-CoV-2 presents a surface “spike” protein that binds to the ACE2 receptor to infect host cells. In addition to the respiratory tract, SARS-Cov-2 can also infect cells of the oral mucosa, which also express the ACE2 receptor. The spike and ACE2 proteins are highly glycosylated with sialic acid modifications that direct viral-host interactions and infection. Maackia amurensis seed lectin (MASL) has a strong affinity for sialic acid modified proteins and can be used as an antiviral agent. Here, we report that MASL targets the ACE2 receptor, decreases ACE2 expression and glycosylation, suppresses binding of the SARS-CoV-2 spike protein, and decreases expression of inflammatory mediators by oral epithelial cells that cause ARDS in COVID-19 patients. In addition, we report that MASL also inhibits SARS-CoV-2 infection of kidney epithelial cells in culture. This work identifies MASL as an agent with potential to inhibit SARS-CoV-2 infection and COVID-19 related inflammatory syndromes.
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DOI：
10.1038/nrmicro.2016.81
发表时间：
2016-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
de Wit E;van Doremalen N;Falzarano D;Munster VJ
通讯作者：
Munster VJ
影响因子：
36.4
作者：
Goetzinger, Florian;Santiago-Garcia, Begona;Tebruegge, Marc
通讯作者：
Tebruegge, Marc
影响因子：
6.4
作者：
Ahsan, Fadhil;Moura-Alves, Pedro;Maertzdorf, Jeroen
通讯作者：
Maertzdorf, Jeroen
影响因子：
--
作者：
Ding L;Wang X;Hong X;Lu L;Liu D
通讯作者：
Liu D
影响因子：
15.1
作者：
Kewan, Tariq;Covut, Fahrettin;Akbik, Bassel
通讯作者：
Akbik, Bassel