Molecular identification of the skin transformation center of anuran larval skin using genes of Rana adult keratin (RAK) and SPARC as probes
Molecular identification of the skin transformation center of anuran larval skin using genes of Rana adult keratin (RAK) and SPARC as probes
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以林蛙成体角蛋白（RAK）和SPARC基因为探针对无尾目幼虫皮肤转化中心进行分子鉴定
DOI：
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
K. Yoshizato
中科院分区：
文献类型：
--
作者：
Y. Ishida;Ken;R. Utoh;M. Obara;K. Yoshizato
无尾两栖类幼体的皮肤经历了一个蜕变成成体前和成体皮肤的过程。基底丝、幼虫基底细胞和成体基底细胞分别是幼虫、前成体和成体表皮中的基底膜附着细胞，并且被鉴定为表达RLK（蛙幼虫角蛋白）、RLK和RAK（蛙成体角蛋白）以及RAK的基因的细胞。幼虫到成体前的皮肤转化发生在称为皮肤转化中心（STC）的组织学实体中。本研究利用cDNA消减法筛选了皮肤组织的cDNA，并克隆了在皮肤组织转化过程中表达上调的分泌型酸性蛋白和富含半胱氨酸基因（secreted protein acidic and rich in cysteine，CIPs）。STC内和周围的RAK基因阳性基底细胞和成纤维细胞分别呈弱和强sparc阳性。使用sparc和rak，我们重新定义了STC，并在组织学切片上将其可视化为约150 μm长的区域，其中包含约20个rak阴性和弱sparc阳性的基底细胞。强烈的sparc表达观察到在基底绞细胞，但不是在幼虫的基底细胞，表明在表皮分化的rak作为一个抑制剂，reflections。这一建议是通过调查的影响，培养的幼虫基底细胞。我们观察到，tetramine抑制rak的表达，但不抑制rlk。
Anuran larval skin undergoes a process of metamorphosis into preadult and adult skin. Basal skein, larval basal and adult basal cells are basement membrane‐attaching cells in the larval, preadult and adult epidermis, respectively, and are identified as cells expressing genes of RLK (Rana larval keratin), both RLK and RAK (Rana adult keratin), and RAK. Larval to preadult skin conversion takes place in the histological entity called the skin transformation center (STC). The present study performed a cDNA subtractive gene screening on cDNA of the larval and the preadult skin, and cloned the secreted protein acidic and rich in cysteine (SPARC) gene as an upregulated gene in the larva to preadult skin conversion. RAK gene‐positive basal skein cells and fibroblasts in and around the STC were weakly and strongly sparc‐positive, respectively. Using sparc and rak, we redefined the STC and visualized it on a histological section as an approximately 150 µm‐long region that contained about 20 rak‐negative and weakly sparc‐positive basal cells. Intense sparc expression was observed in basal skein cells, but not in larval basal cells, suggesting that SPARC acts as a suppressor of rak during epidermal differentiation. This suggestion was tested by investigating the effect of SPARC on cultured larval basal cells. We observed that SPARC suppressed the expression of rak, but not rlk.
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影响因子：
15.9
作者：
Bradshaw, AD;Sage, EH
通讯作者：
Sage, EH
DOI：
10.1073/pnas.260141297
发表时间：
2000-11
影响因子：
11.1
作者：
Laurent M. Sachs;Yun-Bo Shi
通讯作者：
Laurent M. Sachs;Yun-Bo Shi
影响因子：
4.6
作者：
G. Schatteman;Kathleen Morrison-Graham;A. V. Koppen;J. A. Weston;D. F. Bowen-Pope
通讯作者：
G. Schatteman;Kathleen Morrison-Graham;A. V. Koppen;J. A. Weston;D. F. Bowen-Pope
鸡胚 SPARC（骨连接素）的分子分析。
DOI：
10.1111/j.1432-1033.1993.tb18358.x
发表时间：
1993
期刊：
European journal of biochemistry
影响因子：
--
作者：
Bassuk,JA;Iruela-Arispe,ML;Lane,TF;Benson,JM;Berg,RA;Sage,EH
通讯作者：
Sage,EH