Sensitization potential and potency of terpene hydroperoxides in the cocultured activation test method
Sensitization potential and potency of terpene hydroperoxides in the cocultured activation test method
复制标题
共培养活化试验方法中萜烯氢过氧化物的致敏潜力和效力
DOI：
10.1111/cod.13286
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发表时间：
2019
影响因子：
5.5
通讯作者：
Blömeke
中科院分区：
文献类型：
--
作者：
Hennen;Silva e Sousa;Lichter;Lepoittevin;Gimenez-Arnau;Blömeke
背景对含有过敏性氢过氧化物的氧化萜烯混合物的阳性斑贴试验反应经常被报道。目的：利用人细胞分析和评价过氧化氢在体外对人体的致敏潜力和效力。材料/方法：柠檬烯-1-氢过氧化物、柠檬烯-2-氢过氧化物、香茅醇-7-氢过氧化物、枯烯氢过氧化物、1‐（1-氢过氧基-1-甲基乙基）环己烯和香茅醇氢过氧化物的混合物（位置6和7的异构体）和芳樟醇氢过氧化物（位置6和7的异构体）。除了氢过氧化枯烯（其可商购获得）之外，所有化合物均被合成。通过在由HaCaT细胞（人角质形成细胞系）和THP-1单核细胞（作为DC的替代物）组成的共培养活化试验（COCAT）中暴露后测量THP-1细胞上的CD 86和CD 54的上调来评估它们活化树突状细胞（DC）的潜力和效力。结果与其致敏效力排名在预测animal models.ConclusionsFor第一次，人类致敏潜力和效力的几个氢过氧化物测定通过使用人类细胞和COCAT方法。
BackgroundPositive patch test reactions to mixtures of oxidized terpenes containing allergenic hydroperoxides are frequently reported. However, human sensitization data for these hydroperoxides are not available.ObjectivesTo analyse and evaluate the human sensitization potential and potency of hydroperoxides in vitro by using human cells.Materials/MethodsLimonene‐1‐hydroperoxide, limonene‐2‐hydroperoxide, citronellol‐7‐hydroperoxide, cumene hydroperoxide, 1‐(1‐hydroperoxy‐1‐methylethyl)cyclohexene and mixtures of citronellol hydroperoxides (isomers at positions 6 and 7) and linalool hydroperoxides (isomers at positions 6 and 7) were studied. All compounds were synthesized except for cumene hydroperoxide, which was commercially available. Their potential and potency to activate dendritic cells (DCs) was evaluated by measuring the upregulation of CD86 and CD54 on THP‐1 cells upon exposure in the cocultured activation test (COCAT) consisting of HaCaT cells (human keratinocyte cell line) and THP‐1 monocytes (as a surrogate for DCs).ResultsHydroperoxides upregulated CD86 and/or CD54 on cocultured THP‐1 cells in a concentration‐dependent manner. The results are comparable with their sensitization potency ranking in predictive animal models.ConclusionsFor the first time, the human sensitization potential and potency of several hydroperoxides were determined by the use of human cells and the COCAT method.
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