Single-cell transcriptomic profile of satellite glial cells in trigeminal ganglion.
Single-cell transcriptomic profile of satellite glial cells in trigeminal ganglion.
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DOI：
10.3389/fnmol.2023.1117065
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发表时间：
2023
影响因子：
4.8
通讯作者：
Huang, Fang
中科院分区：
文献类型：
--
作者：
Chu, Yanhao;Jia, Shilin;Xu, Ke;Liu, Qing;Mai, Lijia;Liu, Jiawei;Fan, Wenguo;Huang, Fang
关键词：
卫星胶质细胞(SGCs)在三叉神经节(TG)神经元的功能调节中起着重要作用。SGCs与处于不同生理和病理状态的神经元之间的双向通讯涉及多个介质。然而，对SGCs转录本特征的分子洞察是有限的。此外，对TG中SGCs的异质性知之甚少，需要更深入地了解SGCs与神经元亚型之间的相互作用。在这里，我们展示了生理条件下TG中SGCs的单细胞RNA测序(scRNA-seq)谱。结果表明，TG包括神经元、SGCs、髓系雪旺细胞(MSCs)、非髓系雪旺细胞(NMSCs)、免疫细胞等9种类型的细胞团，并给出了相应的标记。我们揭示了SGCs、MSCs和NMSCs在TG中的标志性基因表达，并分析了TG中神经元亚型和SGCs之间的配体-受体对。在SGCs的异质性分析中，确定了SGCs的四种亚型，包括与细胞外基质组织相关的基因富集型、即刻早期基因亚型、干扰素β亚型和细胞黏附分子亚型。我们的数据提示了SGCs的分子特性、异质性以及SGCs与神经元之间的双向相互作用，为研究TG中的SGCs提供了有价值的资源。
Satellite glial cells (SGCs) play an important role in regulating the function of trigeminal ganglion (TG) neurons. Multiple mediators are involved in the bidirectional communication between SGCs and neurons in different physiological and pathological states. However, molecular insights into the transcript characteristics of SGCs are limited. Moreover, little is known about the heterogeneity of SGCs in TG, and a more in-depth understanding of the interactions between SGCs and neuron subtypes is needed. Here we show the single-cell RNA sequencing (scRNA-seq) profile of SGCs in TG under physiological conditions. Our results demonstrate TG includes nine types of cell clusters, such as neurons, SGCs, myeloid Schwann cells (mSCs), non-myeloid Schwann cells (nmSCs), immune cells, etc., and the corresponding markers are also presented. We reveal the signature gene expression of SGCs, mSCs and nmSCs in the TG, and analyze the ligand-receptor pairs between neuron subtypes and SGCs in the TG. In the heterogeneity analysis of SGCs, four SGCs subtypes are identified, including subtypes enriched for genes associated with extracellular matrix organization, immediate early genes, interferon beta, and cell adhesion molecules, respectively. Our data suggest the molecular characteristics, heterogeneity of SGCs, and bidirectional interactions between SGCs and neurons, providing a valuable resource for studying SGCs in the TG.
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影响因子：
11.4
作者：
Fan F;Malvestiti S;Vallet S;Lind J;Garcia-Manteiga JM;Morelli E;Jiang Q;Seckinger A;Hose D;Goldschmidt H;Stadlbauer A;Sun C;Mei H;Pecherstorfer M;Bakiri L;Wagner EF;Tonon G;Sattler M;Hu Y;Tassone P;Jaeger D;Podar K
通讯作者：
Podar K
影响因子：
7.3
作者：
Bonecchi R;Graham GJ
通讯作者：
Graham GJ
影响因子：
6.2
作者：
George, Dale;Ahrens, Paige;Lambert, Stephen
通讯作者：
Lambert, Stephen
影响因子：
16.2
作者：
Eshed, Y;Feinberg, K;Peles, E
通讯作者：
Peles, E
影响因子：
25
作者：
Hasel, Philip;Rose, Indigo V. L.;Liddelow, Shane A.
通讯作者：
Liddelow, Shane A.