Metabolic Engineering of Escherichia coli for Efficient Biosynthesis of Lacto-N-tetraose Using a Novel β-1,3-Galactosyltransferase from Pseudogulbenkiania ferrooxidans.
Metabolic Engineering of Escherichia coli for Efficient Biosynthesis of Lacto-N-tetraose Using a Novel β-1,3-Galactosyltransferase from Pseudogulbenkiania ferrooxidans.
复制标题
使用来自 Pseudogulbenkiania 氧化亚铁的新型 β-1,3-半乳糖基转移酶对大肠杆菌进行代谢工程，以高效生物合成乳-N-四糖。
DOI：
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发表时间：
2021
影响因子：
6.1
通讯作者：
W. Mu
中科院分区：
文献类型：
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作者：
Yingying Zhu;Zeyu Li;Guocong Luo;Hao Wu;Wenli Zhang;W. Mu
近年来，母乳低聚糖（HMO）因其对婴儿健康的特殊作用而引起了人们的极大兴趣。乳糖-N-四糖（LNT）是最丰富的HMO之一，已在商业上作为功能强化剂添加到婴儿配方奶粉中。本研究从14株氧化亚铁拟古菌中筛选出一株产LNT的β-1，3-半乳糖基转移酶（β-1，3-GalT），并在此基础上，通过加强UDP-半乳糖合成和β-1，3-GalT的导入，构建了一株高效产LNT的大肠杆菌代谢工程菌。工程菌摇瓶发酵和流加发酵的LNT产量分别为3.11和25.49 g/L，LNT II转化率分别为88.15%和85.09%。流加培养的LNT生产能力为0.61 g/L·h，比产量为0.76 g/g干细胞。据我们所知，这是有史以来报道的最高LNT产量。
Human milk oligosaccharides (HMOs) attract considerable interest in recent years because of their particular role in infant health. Lacto-N-tetraose (LNT), one of the most abundant HMOs, has been commercially added in the infant formula as a functional fortifier. In this study, a novel LNT-producing β-1,3-galactosyltransferase (β-1,3-GalT) from Pseudogulbenkiania ferrooxidans was screened from 14 putative candidates, and a highly LNT-producing metabolically engineered Escherichia coli strain was constructed based on a previously constructed lacto-N-triose II (LNT II)-producing strain, by strengthening UDP-galactose synthesis and introduction of P. ferrooxidans β-1,3-GalT. The engineered strain produced 3.11 and 25.49 g/L LNT in shake-flask and fed-batch cultivation, with the molar conversion ratio of LNT II to LNT of 88.15 and 85.09%, respectively. The productivity and specific yield of LNT in fed-batch cultivation were measured to be 0.61 g/L·h and 0.76 g/g dry cell weight, respectively. To the best of our knowledge, it is the highest LNT yield ever reported.
影响因子：
5.3
作者：
Moore RE;Xu LL;Townsend SD
通讯作者：
Townsend SD
影响因子：
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作者：
Chen, Xi
通讯作者：
Chen, Xi
影响因子：
12.9
作者：
McArthur JB;Yu H;Chen X
通讯作者：
Chen X