The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts.
The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts.
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Crenarchaeon sulfolobus solfataricus的SMAP1/2蛋白与外泌体相互作用，并刺激转录物的A含量丰富。
DOI：
10.1093/nar/gkx437
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发表时间：
2017-07-27
影响因子：
14.9
通讯作者：
Bläsi U
中科院分区：
文献类型：
--
作者：
Märtens B;Hou L;Amman F;Wolfinger MT;Evguenieva-Hackenberg E;Bläsi U
保守的Sm和Sm样蛋白参与RNA代谢的不同方面。本研究探讨了绿古菌（Sulfolobus solfataricus, Sso）中SmAP1和SmAP2的相互作用，以阐明它们的生理功能。SmAP1和SmAP2与参与RNA加工/修饰、翻译和蛋白质周转的几种蛋白以及参与RNA 3΄至5΄降解的外泌体组分共同纯化。在后续研究中，证实了与poly(a)结合和辅助外泌体亚基DnaG的直接相互作用。此外，这两种SmAPs水平的升高导致可溶性外泌体部分丰度的增加，表明它们影响外泌体在细胞中的亚细胞定位。外泌体溶解度的增加伴随着富含a尾部的rna水平的增加，这些rna通过RNASeq进一步表征。因此，Sso SmAPs对外泌体活性的影响揭示了SmAPs在古细菌中迄今未被认识的功能。
The conserved Sm and Sm-like proteins are involved in different aspects of RNA metabolism. Here, we explored the interactome of SmAP1 and SmAP2 of the crenarchaeon Sulfolobus solfataricus (Sso) to shed light on their physiological function(s). Both, SmAP1 and SmAP2 co-purified with several proteins involved in RNA-processing/modification, translation and protein turnover as well as with components of the exosome involved in 3΄ to 5΄ degradation of RNA. In follow-up studies a direct interaction with the poly(A) binding and accessory exosomal subunit DnaG was demonstrated. Moreover, elevated levels of both SmAPs resulted in increased abundance of the soluble exosome fraction, suggesting that they affect the subcellular localization of the exosome in the cell. The increased solubility of the exosome was accompanied by augmented levels of RNAs with A-rich tails that were further characterized using RNASeq. Hence, the observation that the Sso SmAPs impact on the activity of the exosome revealed a hitherto unrecognized function of SmAPs in archaea.
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DOI：
10.1073/pnas.011535498
发表时间：
2001-01-02
影响因子：
11.1
作者：
Liou, GG;Jane, WN;Lin-Chao, S
通讯作者：
Lin-Chao, S
影响因子：
4.1
作者：
Hou L;Klug G;Evguenieva-Hackenberg E
通讯作者：
Evguenieva-Hackenberg E
影响因子：
4.8
作者：
Fischer, Susan;Benz, Juliane;Marchfelder, Anita
通讯作者：
Marchfelder, Anita
影响因子：
14.9
作者：
Ito K;Honda T;Suzuki T;Miyoshi T;Murakami R;Yao M;Uchiumi T
通讯作者：
Uchiumi T
影响因子：
12.3
作者：
Aravind L;Iyer LM;Anantharaman V
通讯作者：
Anantharaman V