The two faces of Alba: the evolutionary connection between proteins participating in chromatin structure and RNA metabolism.
The two faces of Alba: the evolutionary connection between proteins participating in chromatin structure and RNA metabolism.
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DOI：
10.1186/gb-2003-4-10-r64
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发表时间：
2003
期刊：
影响因子：
12.3
通讯作者：
Anantharaman V
中科院分区：
文献类型：
--
作者：
Aravind L;Iyer LM;Anantharaman V
染色体蛋白的Alba超家族似乎起源于rna结合蛋白，并且可能仅在crenarchaeal谱系中被招募到染色体中。在古细菌中，主要染色体dna结合蛋白的种类模式具有相当大的异质性。Alba是来自crenarchaeal Sulfolobus属的一种具有良好特征的染色体蛋白。虽然在大多数古细菌和一些真核生物分类群中发现了阿尔巴蛋白，但其在这些分类群中的确切功能尚不清楚。在这里，我们使用比较基因组学和序列谱分析来预测Alba蛋白的潜在替代功能。通过序列谱搜索，我们能够将Alba蛋白与RNase P/MRP亚基Rpp20/Pop7、人类RNase P亚基Rpp25和与大核发育有关的Mdp2蛋白统一起来。Alba超家族包含两个真核生物特异性家族和一个古细菌家族。我们提出不同的证据线，以表明这两个真核家族执行功能相关的RNA代谢。真核生物家族的几个成员，以Mdp2为典型，与rna结合的RGG重复序列结合在同一多肽中。我们还研究了统一的Alba超家族在古rna结合IF3-C褶皱中的关系，并表明它与该褶皱的其他rna结合成员（如YhbY和IF3-C超家族）最密切相关。基于系统发育模式和系统发育包络原理，我们预测至少一些古细菌成员也可能在RNA代谢中发挥作用。Alba超家族蛋白似乎起源于形成各种核糖核蛋白复合物的rna结合蛋白，可能包括RNase P.它可能仅在crenarchaeal谱系中被募集为染色体蛋白。这里报道的进化联系表明，基于共同生化基础的功能多样性如何出现在Alba超家族的蛋白质中。
The Alba superfamily of chromosomal proteins appear to have originated as RNA-binding proteins and to have been recruited to chromosomes possibly only within the crenarchaeal lineage. There is considerable heterogeneity in the phyletic patterns of major chromosomal DNA-binding proteins in archaea. Alba is a well-characterized chromosomal protein from the crenarchaeal genus Sulfolobus. While Alba has been detected in most archaea and some eukaryotic taxa, its exact functions in these taxa are not clear. Here we use comparative genomics and sequence profile analysis to predict potential alternative functions of the Alba proteins. Using sequence-profile searches, we were able to unify the Alba proteins with RNase P/MRP subunit Rpp20/Pop7, human RNase P subunit Rpp25, and the ciliate Mdp2 protein, which is implicated in macronuclear development. The Alba superfamily contains two eukaryote-specific families and one archaeal family. We present different lines of evidence to show that both eukaryotic families perform functions related to RNA metabolism. Several members of one of the eukaryotic families, typified by Mdp2, are combined in the same polypeptide with RNA-binding RGG repeats. We also investigated the relationships of the unified Alba superfamily within the ancient RNA-binding IF3-C fold, and show that it is most closely related to other RNA-binding members of this fold, such as the YhbY and IF3-C superfamilies. Based on phyletic patterns and the principle of phylogenetic bracketing, we predict that at least some of the archaeal members may also possess a role in RNA metabolism. The Alba superfamily proteins appear to have originated as RNA-binding proteins which formed various ribonucleoprotein complexes, probably including RNase P. It was recruited as a chromosomal protein possibly only within the crenarchaeal lineage. The evolutionary connections reported here suggest how a diversity of functions based on a common biochemical basis emerged in proteins of the Alba superfamily.
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影响因子：
6.8
作者：
Aravind, L;Mazumder, R;Koonin, EV
通讯作者：
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影响因子：
14.9
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DOI：
10.1016/0167-4781(88)90035-8
发表时间：
1988-11-10
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
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作者：
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