Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae.
Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae.
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DOI：
10.1016/j.jprot.2009.12.001
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发表时间：
2010-03-10
影响因子：
3.3
通讯作者：
Jennings MP
中科院分区：
文献类型：
--
作者：
Wu HJ;Seib KL;Srikhanta YN;Edwards J;Kidd SP;Maguire TL;Hamilton A;Pan KT;Hsiao HH;Yao CW;Grimmond SM;Apicella MA;McEwan AG;Wang AH;Jennings MP
Neisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defense mechanisms that are dependent on manganese (Mn). We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag [ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype. These studies revealed that 97 proteins are regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (eg. Pilin, a key adhesin), oxidative stress defence (eg. superoxide dismutase), cellular metabolism, protein synthesis, RNA processing and cell division. Mn regulation of inorganic pyrophosphatase (Ppa) indicated the potential involvement of phosphate metabolism in the Mn-dependent oxidative stress defense. A detailed analysis of the role of Ppa and polyphosphate kinase (Ppk) in the gonococcal oxidative stress response revealed that ppk and ppa mutant strains showed increased resistance to oxidative stress. Investigation of these mutants grown with high Mn suggests that phosphate and pyrophosphate are involved in Mn-dependent oxidative stress resistance.
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影响因子：
2.8
作者：
Chen, CY;Morse, SA
通讯作者：
Morse, SA
影响因子：
3.2
作者：
ARCHIBALD, FS;FRIDOVICH, I
通讯作者：
FRIDOVICH, I
影响因子：
2.9
作者：
HEINONEN, JK;LAHTI, RJ
通讯作者：
LAHTI, RJ
影响因子：
3.9
作者：
ARCHIBALD, FS;FRIDOVICH, I
通讯作者：
FRIDOVICH, I
影响因子：
3.1
作者：
BUCHANAN, TM;PEARCE, WA
通讯作者：
PEARCE, WA