Adaptation of laser interstitial thermal therapy for tumor ablation under MRI monitoring in a rat orthotopic model of glioblastoma.
Adaptation of laser interstitial thermal therapy for tumor ablation under MRI monitoring in a rat orthotopic model of glioblastoma.
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DOI：
10.1007/s00701-021-05002-y
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发表时间：
2021-12
影响因子：
2.4
通讯作者：
Lee IY
中科院分区：
文献类型：
--
作者：
Nagaraja TN;Bartlett S;Farmer KG;Cabral G;Knight RA;Valadie OG;Brown SL;Ewing JR;Lee IY
磁共振成像（MRI）监测下的激光间质热治疗（LITT）越来越多地用于复发性脑肿瘤和功能区肿瘤的细胞减灭术。本研究的目的是使该技术适用于动物胶质瘤模型。采用U251胶质母细胞瘤（GBM）大鼠模型。通过MRI和动态增强（DCE）MRI确定肿瘤的位置和范围。评估肿瘤外观后一天，使用Visualase LITT系统在扩散加权成像（DWI）-MRI期间消融肿瘤（n=5）。在消融后立即获得脑图像，并在消融后24小时再次获得脑图像，以确认肿瘤细胞消融的有效性。未处理的肿瘤作为对照（n=3）。大鼠注射荧光异硫氰酸酯（FITC）葡聚糖和伊文思蓝循环10分钟后，后LITT MRI。然后取出大脑进行荧光显微镜检查，并使用苏木精和伊红（H&E）和主要组织相容性复合体（MHC）染色进行组织病理学评价。所有大鼠在预LITT成像时均显示T2和T1对比增强的占位性肿瘤。接受LITT手术的大鼠显示出边界清晰的消融区，肿瘤组织几乎完全消融，消融后24小时消融周围造影剂增强。通过H&E和MHC染色证实如在MRI上所见的通过消融的肿瘤细胞减少。数据显示，在临床前胶质瘤模型中，使用MRI监测的LITT进行肿瘤细胞消融是可能的。实时MRI监测有助于可视化和控制消融区域，因为它在临床应用中以其他方式进行。
Laser interstitial thermal therapy (LITT) under magnetic resonance imaging (MRI) monitoring is being increasingly used in cytoreductive surgery of recurrent brain tumors and tumors located in eloquent brain areas. The objective of this study was to adapt this technique to an animal glioma model. A rat model of U251 glioblastoma (GBM) was employed. Tumor location and extent were determined by MRI and dynamic contrast-enhanced (DCE) MRI. A day after assessing tumor appearance, tumors were ablated during diffusion-weighted imaging (DWI)-MRI using a Visualase LITT system (n=5). Brain images were obtained immediately after ablation and again at 24 h post-ablation to confirm the efficacy of tumor cytoablation. Untreated tumors served as controls (n=3). Rats were injected with fluorescent isothiocyanate (FITC) dextran and Evans blue that circulated for 10 min after post-LITT MRI. The brains were then removed for fluorescence microscopy and histopathology evaluations using hematoxylin and eosin (H&E) and major histocompatibility complex (MHC) staining. All rats showed a space-occupying tumor with T2 and T1 contrast-enhancement at pre-LITT imaging. The rats that underwent the LITT procedure showed a well demarcated ablation zone with near-complete ablation of tumor tissue and with peri-ablation contrast enhancement at 24 h post-ablation. Tumor cytoreduction by ablation as seen on MRI was confirmed by H&E and MHC staining. Data showed that tumor cytoablation using MRI-monitored LITT was possible in pre-clinical glioma models. Real-time MRI monitoring facilitated visualizing and controlling the area of ablation as it is otherwise performed in clinical applications.
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影响因子：
2.9
作者：
Ewing JR;Nagaraja TN;Aryal MP;Keenan KA;Elmghirbi R;Bagher-Ebadian H;Panda S;Lu M;Mikkelsen T;Cabral G;Brown SL
通讯作者：
Brown SL
影响因子：
2.4
作者：
Carpentier, Alexandre;Chauvet, Dorian;Delattre, Jean-Yves
通讯作者：
Delattre, Jean-Yves
影响因子：
2.9
作者：
Nagaraja TN;Elmghirbi R;Brown SL;Rey JA;Schultz L;Mukherjee A;Cabral G;Panda S;Lee IY;Sarntinoranont M;Keenan KA;Knight RA;Ewing JR
通讯作者：
Ewing JR
影响因子：
2.4
作者：
Oertel MF;Stieglitz LH;Bozinov O
通讯作者：
Bozinov O
影响因子：
3.7
作者：
Emerich, DF;Winn, SR;Bartus, RT
通讯作者：
Bartus, RT