Single-cell transcriptome analysis of the zebrafish embryonic trunk.

Single-cell transcriptome analysis of the zebrafish embryonic trunk.
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DOI:
10.1371/journal.pone.0254024
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发表时间:
2021
期刊:
影响因子:
3.7
通讯作者:
Sumanas S
Sumanas S
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Metikala S;Casie Chetty S;Sumanas S

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在胚胎发育过程中,细胞分化成具有不同转录谱的不同细胞类型和亚型。迄今为止,在发育过程中出现的不同细胞系的转录组特征仅被部分表征。在这里,我们使用单细胞RNA-seq对30 hpf期斑马鱼胚胎躯干区域分解的超过20,000个细胞进行转录组学分析。在分析过程中,鉴定并注释了27种不同细胞类型和亚型的转录特征。该数据集将为发育和细胞生物学领域的许多研究人员提供有用的资源,并促进对发育过程中调节细胞谱系选择的分子机制的理解。
During embryonic development, cells differentiate into a variety of distinct cell types and subtypes with diverse transcriptional profiles. To date, transcriptomic signatures of different cell lineages that arise during development have been only partially characterized. Here we used single-cell RNA-seq to perform transcriptomic analysis of over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage. Transcriptional signatures of 27 different cell types and subtypes were identified and annotated during this analysis. This dataset will be a useful resource for many researchers in the fields of developmental and cellular biology and facilitate the understanding of molecular mechanisms that regulate cell lineage choices during development.
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