Co-Cr-Mo alloy particles induce tumor necrosis factor alpha production in MLO-Y4 osteocytes: a role for osteocytes in particle-induced inflammation.
Co-Cr-Mo alloy particles induce tumor necrosis factor alpha production in MLO-Y4 osteocytes: a role for osteocytes in particle-induced inflammation.
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DOI：
10.1016/j.bone.2009.05.020
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发表时间：
2009-09
期刊：
影响因子：
4.1
通讯作者：
Sena, Kotaro
中科院分区：
文献类型：
--
作者：
Kanaji, Arihiko;Caicedo, Marco S.;Virdi, Amarjit S.;Sumner, D. Rick;Hallab, Nadim J.;Sena, Kotaro
磨损碎屑引起的骨溶解据称是影响关节置换术长期结果的限制性问题。磨损碎屑在种植体周围细胞如巨噬细胞、成骨细胞和破骨细胞中的致病作用已得到充分研究。相比之下，磨损碎片对骨细胞的影响仍然未知，骨细胞占所有骨细胞的90%以上。我们假设金属植入物碎片可以诱导骨细胞的促炎反应。本研究证明了钴-铬-钼合金（Co-Cr-Mo）颗粒对充分表征的MLO-Y 4骨细胞系的影响。Co-Cr-Mo合金颗粒处理3、6 h后显著上调骨细胞TNFα基因表达（p<0.05），24 h后显著上调TNFα蛋白表达（p<0.05），6 h后显著下调IL-6基因表达（p <0.05），24 h后诱导骨细胞凋亡，这种凋亡效应部分依赖于TNFα（40%）。因此，我们的研究结果表明，骨细胞通过诱导促炎细胞因子和诱导骨细胞凋亡在颗粒诱导的炎症和骨吸收全髋关节置换术后发挥作用。
Wear debris-induced osteolysis is purportedly the limiting problem affecting the long term results of joint arthroplasty. Pathogenic effects of wear debris in peri-implant cells such as macrophages, osteoblasts and osteoclasts have been well studied. In contrast, the affects of wear-debris on osteocytes, which make up over 90% of all bone cells, remains unknown. We hypothesized that metal implant debris can induce the proinflammatory response in osteocytes. This study demonstrated the effects of cobalt-chromium-molybdenum alloy (Co-Cr-Mo) particles on a well-characterized MLO-Y4 osteocyte cell line. Co-Cr-Mo alloy particle treatment significantly (p<0.05) up-regulated tumor necrosis factor alpha (TNFα) gene expression after 3 and 6 hr and TNFα protein production after 24 hr, but down-regulated interleukin-6 (IL-6) gene expression after 6 hr. Co-Cr-Mo alloy particle treatment also induced osteocyte apoptosis after 24 hr. This apoptotic effect was partially (40%) dependent on TNFα. Therefore, our results suggest that osteocytes play a role in particle induced inflammation and bone resorption following total hip arthroplasty by inducing pro-inflammatory cytokines and inducing osteocyte apoptosis.
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影响因子：
4.4
作者：
Doodes, Paul D.;Cao, Yanxia;Hamel, Keith M.;Wang, Yumei;Farkas, Balint;Iwakura, Yoichiro;Finnegan, Alison
通讯作者：
Finnegan, Alison
DOI：
10.2106/00004623-200112000-00004
发表时间：
2001-12-01
影响因子：
5.3
作者：
Childs, LM;Goater, JJ;Schwarz, EM
通讯作者：
Schwarz, EM
影响因子：
5.3
作者：
Jacobs, JJ;Hallab, NJ;Wimmer, MA
通讯作者：
Wimmer, MA
影响因子：
2.9
作者：
Sena, Kotaro;Sumner, Dale R.;Virdi, Amarjit S.
通讯作者：
Virdi, Amarjit S.
影响因子：
2.8
作者：
Schwarz, EM;Lu, AP;O'Keefe, RJ
通讯作者：
O'Keefe, RJ