DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism.
DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism.
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DA-6 通过介导脂肪酸代谢和糖代谢促进老化大豆种子的发芽和成苗
DOI：
10.1093/jxb/ery247
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发表时间：
2019-01-01
影响因子：
6.9
通讯作者：
Shu K
中科院分区：
文献类型：
--
作者：
Zhou W;Chen F;Zhao S;Yang C;Meng Y;Shuai H;Luo X;Dai Y;Yin H;Du J;Liu J;Fan G;Liu W;Yang W;Shu K
植物生长调节剂二乙基氨乙基己酸酯（DA-6）通过促进三酰甘油向脂肪酸和糖的转化来促进老化大豆种子的萌发和成苗。大豆种子比谷类作物种子含有更高浓度的油（三酰甘油）和脂肪酸，这些生物分子在种子储存期间的氧化显着缩短种子寿命并降低发芽能力。在这里，我们报告，二乙基氨基乙基己酸（DA-6），植物生长调节剂，增加发芽和幼苗建立从老化的大豆种子，通过增加脂肪酸代谢和糖代谢。表型分析表明，DA-6处理显著促进自然老化和人工老化大豆种子的萌发和成苗。进一步分析表明，DA-6增加了老化大豆种子吸胀过程中可溶性糖的浓度。DA-6处理的老化种子中几种不同脂肪酸的含量均高于未处理的老化种子。随后，定量PCR分析表明，DA-6诱导老化大豆种子中参与三酰甘油水解为糖的几个关键基因的转录。此外，老化种子中的转化酶活性，催化蔗糖水解形成果糖和葡萄糖，增加后DA-6处理。总之，DA-6通过增强三酰甘油的水解和脂肪酸向糖的转化来促进老化大豆种子的萌发和幼苗建立。
The plant growth regulator diethyl aminoethyl hexanoate (DA-6) promotes the germination and seedling establishment of aged soybean seeds through enhancing the conversion from triacylglycerol to fatty acids and sugars. Soybean seeds contain higher concentrations of oil (triacylglycerol) and fatty acids than do cereal crop seeds, and the oxidation of these biomolecules during seed storage significantly shortens seed longevity and decreases germination ability. Here, we report that diethyl aminoethyl hexanoate (DA-6), a plant growth regulator, increases germination and seedling establishment from aged soybean seeds by increasing fatty acid metabolism and glycometabolism. Phenotypic analysis showed that DA-6 treatment markedly promoted germination and seedling establishment from naturally and artificially aged soybean seeds. Further analysis revealed that DA-6 increased the concentrations of soluble sugars during imbibition of aged soybean seeds. Consistently, the concentrations of several different fatty acids in DA-6-treated aged seeds were higher than those in untreated aged seeds. Subsequently, quantitative PCR analysis indicated that DA-6 induced the transcription of several key genes involved in the hydrolysis of triacylglycerol to sugars in aged soybean seeds. Furthermore, the activity of invertase in aged seeds, which catalyzes the hydrolysis of sucrose to form fructose and glucose, increased following DA-6 treatment. Taken together, DA-6 promotes germination and seedling establishment from aged soybean seeds by enhancing the hydrolysis of triacylglycerol and the conversion of fatty acids to sugars.
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