Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed.
Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed.
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DOI：
10.1111/j.1467-7652.2011.00650.x
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发表时间：
2012-02
影响因子：
13.8
通讯作者：
Trick HN
中科院分区：
文献类型：
--
作者：
Lee J;Welti R;Roth M;Schapaugh WT;Li J;Trick HN
磷脂组成的变化和随之而来的膜完整性的丧失与种子活力的丧失有关。此外，磷脂组成的变化会影响三酰甘油的组成，即储存脂。磷脂酶D(Pld)催化磷脂水解物生成磷脂酸，而pldα是一种丰富的pld亚型。虽然储存33个月的野生型种子是不活的，但储存33个月的α-Knkdown(PLD-KD)大豆种子有30%~50%发芽。在老化过程中，野生型和PLD-KD种子的溶血磷脂水平和三酰甘油脂肪酸不饱和度均有所增加，但野生型的增加幅度大于PLD-KD种子。野生型种子活力的丧失与超微结构的变化有关，包括质膜从细胞壁复合体中脱落和油体解体。这些数据表明，在自然老化过程中，磷脂酶Dα影响大豆磷脂和三酰甘油的组成。抑制大豆种子中PLD活性对提高种子长期贮藏品质具有重要意义。
Changes in phospholipid composition and consequent loss of membrane integrity are correlated with loss of seed viability. Furthermore, phospholipid compositional changes affect the composition of the triacylglycerols, i.e. the storage lipids. Phospholipase D (PLD) catalyzes the hydrolysis of phospholipids to phosphatidic acid, and PLDα is an abundant PLD isoform. Although wild type seeds stored for 33 months were non-viable, 30 to 50% of PLDα-knockdown (PLD-KD) soybean seeds stored for 33 months germinated. Wild type and PLD-KD seeds increased in lysophospholipid levels and in triacylglycerol fatty acid unsaturation during aging, but the levels of lysophospholipids increased more in wild type than in PLD-KD seeds. The loss of viability of wild type seeds was correlated with alterations in ultrastructure, including detachment of the plasma membrane from the cell wall complex and disorganization of oil bodies. The data demonstrate that, during natural aging, PLDα affects the soybean phospholipid profile and the triacylglycerol profile. Suppression of PLD activity in soybean seed has potential for improving seed quality during long-term storage.
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