BISMA--fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences.
BISMA--fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences.
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DOI：
10.1186/1471-2105-11-230
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发表时间：
2010-05-06
影响因子：
3
通讯作者：
Jeltsch A
中科院分区：
文献类型：
--
作者：
Rohde C;Zhang Y;Reinhardt R;Jeltsch A
亚硫酸氢盐测序是一种流行的高分辨率DNA甲基化模式分析方法。聚合酶链式反应针对一个感兴趣的区域，通常分析大约20-50个亚克隆DNA分子，以确定单个CpG位点的甲基化状态和分子分辨率。用于分析初级亚硫酸氢盐测序数据的BISMA(亚硫酸氢盐测序DNA甲基化分析)软件实现了测序数据提取和增强的数据处理、质量控制、甲基化状态的分析和呈现。它使用一种改进的策略来检测克隆分子和准确的CpG位点检测，并首次支持重复序列的分析。BISMA工作高度自动化，但仍为用户提供对分析所有步骤的完全控制。BISMA软件可免费作为学术用途的在线工具，用于分析来自唯一和重复序列http://biochem.jacobs-university.de/BDPC/BISMA/.的亚硫酸氢盐测序数据
Bisulfite sequencing is a popular method to analyze DNA methylation patterns at high resolution. A region of interest is targeted by PCR and about 20-50 subcloned DNA molecules are usually analyzed, to determine the methylation status at single CpG sites and molecule resolution. The BISMA (Bisulfite Sequencing DNA Methylation Analysis) software for analysis of primary bisulfite sequencing data implements sequencing data extraction and enhanced data processing, quality controls, analysis and presentation of the methylation state. It uses an improved strategy for detection of clonal molecules and accurate CpG site detection and it supports for the first time analysis of repetitive sequences. BISMA works highly automated but still provides the user full control over all steps of the analysis. The BISMA software is freely available as an online tool for academic purposes for the analysis of bisulfite sequencing data from both unique and repetitive sequences http://biochem.jacobs-university.de/BDPC/BISMA/.
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影响因子：
4.4
作者：
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通讯作者：
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