Heterogeneous regulation of Staphylococcus aureus by different Staphylococcus epidermidis agr types in atopic dermatitis.
Heterogeneous regulation of Staphylococcus aureus by different Staphylococcus epidermidis agr types in atopic dermatitis.
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特应性皮炎中不同表皮葡萄球菌 agr 类型对金黄色葡萄球菌的异质调节。
DOI：
10.1016/j.jid.2023.05.014
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
X. Yao
中科院分区：
文献类型：
--
作者：
Yuan;Xiaoqiang Xu;Yang Liu;Ao Wang;Yang Luo;Xiaochun Liu;Xiaokai Wang;Wei Li;X. Yao
表皮葡萄球菌在皮肤炎症中具有保护作用，但其在特应性皮炎（AD）中的确切作用及其机制尚不完全清楚。在此，对分离自儿童AD患者的表皮葡萄球菌菌株进行全基因组测序，发现功能基因在菌株水平上存在显著异质性。对表皮葡萄球菌的特异性序列分析根据agr基因座的变异，鉴定出4种类型的辅助基因调节子（agr），这与环境微生物群的宏基因组数据相一致。AD分离株中I型表皮葡萄球菌的数量略有减少，而IV型几乎未检测到。功能实验表明，I型和IV型表皮葡萄球菌菌株（而非II型和III型）在体外抑制金黄色葡萄球菌介导的毒力因子的表达，抑制金黄色葡萄球菌表皮定植，并减轻小鼠模型中的皮肤炎症。AD患者表皮葡萄球菌菌株水平的基因组特征以及IV型表皮葡萄球菌和金黄色葡萄球菌之间的群体感应干扰为调节皮肤微生物群和治疗AD提供了基础。
The skin commensalStaphylococcus epidermidisexhibits a protective role in skin inflammation; however, the exact functions ofS. epidermidisand their mechanisms in atopic dermatitis (AD) are not fully understood. Here, whole-genome sequencing was conducted on strains ofS. epidermidisisolated from pediatric patients with AD and revealed significant strain-level heterogeneity in functional genes. Specific sequence analysis ofS. epidermidisidentified four types of accessory gene regulator (agr) according to locus variations in theagroperon, which was consistent with the metagenomic data of the contextual microbiota. The number ofS. epidermidisagrtype I was slightly decreased among AD isolates, whereasagrtype IV was hardly detected in AD isolates. Functional experiments showed that strains ofS. epidermidisagrtypes I and IV, but not types II and III, inhibited the expression ofS. aureusagr-mediated virulence factors in vitro, suppressedS. aureusepidermal colonization, and attenuated skin inflammation in a mouse model. The delineation of genome signatures ofS. epidermidisat the strain level in AD and the quorum-sensing interference betweenS. epidermidisagrtype IV andS. aureusprovide a foundation for the modulation of the skin microbiota and the treatment of AD.
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影响因子：
30.3
作者：
Liu H;Archer NK;Dillen CA;Wang Y;Ashbaugh AG;Ortines RV;Kao T;Lee SK;Cai SS;Miller RJ;Marchitto MC;Zhang E;Riggins DP;Plaut RD;Stibitz S;Geha RS;Miller LS
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Miller LS
影响因子：
62.1
作者：
Thoendel, Matthew;Kavanaugh, Jeffrey S.;Flack, Caralyn E.;Horswill, Alexander R.
通讯作者：
Horswill, Alexander R.
影响因子：
30.3
作者：
Williams MR;Nakatsuji T;Gallo RL
通讯作者：
Gallo RL
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Y. Nakamura;J. Oscherwitz;K. Cease;Susana M. Chan;Raúl Muñoz-Planillo;M. Hasegawa;A. Villaruz;Gordon Y. C. Cheung;M. McGavin;J. Travers;M. Otto;N. Inohara;G. Núñez;M. J. M. A. E. Generated-M.-J.-M.-A.-E.-Generated-2252430255;M. O. Engineered
通讯作者：
Y. Nakamura;J. Oscherwitz;K. Cease;Susana M. Chan;Raúl Muñoz-Planillo;M. Hasegawa;A. Villaruz;Gordon Y. C. Cheung;M. McGavin;J. Travers;M. Otto;N. Inohara;G. Núñez;M. J. M. A. E. Generated-M.-J.-M.-A.-E.-Generated-2252430255;M. O. Engineered
影响因子：
30.3
作者：
Zheng Y;Hunt RL;Villaruz AE;Fisher EL;Liu R;Liu Q;Cheung GYC;Li M;Otto M
通讯作者：
Otto M