Heterogeneous multi-compartmental hydrogel particles as synthetic cells for incompatible tandem reactions.
Heterogeneous multi-compartmental hydrogel particles as synthetic cells for incompatible tandem reactions.
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DOI：
10.1038/s41467-017-00757-4
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发表时间：
2017-09-22
影响因子：
16.6
通讯作者：
Chen CH
中科院分区：
文献类型：
--
作者：
Tan H;Guo S;Dinh ND;Luo R;Jin L;Chen CH
在自然界中，单个细胞包含多个隔离的隔室，其中发生级联酶促反应以高效地形成必需的生物产物。在这里，我们报告了一种具有多个隔室的功能性水凝胶颗粒的细胞灵感设计，其中不同的酶在空间上固定在不同的结构域中，从而实现工程化的一锅法串联反应。水凝胶颗粒中不同隔室的致密填充使得能够有效地输送反应物，以确保以高效率产生产物。为了证明微环境修饰的优点，我们采用丙烯酸的共聚，这导致形成具有不同pH微环境的异质多室水凝胶颗粒。在葡萄糖氧化酶和磁性纳米颗粒的位置组装后，这些水凝胶颗粒能够在一锅中处理葡萄糖触发的、不相容的多步串联反应。此外，基于羟基自由基的高细胞毒性，探讨了葡萄糖驱动的杀死癌细胞的治疗策略。细胞包含分离的区室，在其中发生级联酶生化反应以高效地形成必需的生物产物。在这里，作者通过微流体技术生产具有多个隔室的功能性水凝胶颗粒，这些微流体在不同的域中含有空间固定的天然酶，用于一锅串联反应。
In nature, individual cells contain multiple isolated compartments in which cascade enzymatic reactions occur to form essential biological products with high efficiency. Here, we report a cell-inspired design of functional hydrogel particles with multiple compartments, in which different enzymes are spatially immobilized in distinct domains that enable engineered, one-pot, tandem reactions. The dense packing of different compartments in the hydrogel particle enables effective transportation of reactants to ensure that the products are generated with high efficiency. To demonstrate the advantages of micro-environmental modifications, we employ the copolymerization of acrylic acid, which leads to the formation of heterogeneous multi-compartmental hydrogel particles with different pH microenvironments. Upon the positional assembly of glucose oxidase and magnetic nanoparticles, these hydrogel particles are able to process a glucose-triggered, incompatible, multistep tandem reaction in one pot. Furthermore, based on the high cytotoxicity of hydroxyl radicals, a glucose-powered therapeutic strategy to kill cancer cells was approached. Cells contain isolated compartments where cascade enzymatic biochemical reactions occur to form essential biological products with high efficiency. Here the authors produce functional hydrogel particles with multiple compartments via microfluidics that contain spatially immobilized natural enzymes in distinct domains for one-pot, tandem reactions.
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