Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection.
Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection.
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抗病基因的鉴定及其在抗病育种中的应用是解决对虾养殖病害问题的重要途径。虽然已经进行了一些转录组学研究来鉴定与病原体感染和宿主防御相关的基因，但仍然缺乏对副溶血性弧菌感染的宿主抗性特征的深入了解。本研究建立了一种获得抗副溶血性弧菌感染的对虾血细胞的方法。我们还提出了一个策略，以确定关键的生物学过程和抗病相关基因的基础上分析目前的转录组数据。副溶血性弧菌（Vibrio parahaemolyticus）携带的质粒编码毒素PirA和PirB是导致对虾养殖中严重AHPND的病原之一。然而，人们对副溶血性弧菌感染的宿主耐药特征缺乏深入了解。本研究建立了一种从具有不同副溶血性弧菌抗性的对虾中获得血细胞的方法，并对两个独立群体中对虾血细胞背景水平的表达谱进行了比较转录组分析。主成分分析和聚类分析结果表明，来自同一群体的样品间的亲缘关系较近，而来自抗病能力相近的对虾样品间的亲缘关系较远。DEGs分析表明，两个群体之间的DEGs数量远多于副溶血性弧菌抗性虾和敏感虾之间的DEGs数量。通过对来自群体1和2的副溶血性弧菌抗性虾和敏感虾的比较，分别鉴定出31个DEG和5个DEG。群体1的DEG基因主要为细胞因子相关基因、代谢相关基因和免疫相关基因。虽然两个比较之间没有DEG重叠，但来自群体2的DEG还包括与细胞骨架和代谢相关的基因。这些数据表明，这些生物学过程在抗病性中起着重要作用，可以通过对多组学数据的综合分析来关注它们。本研究为筛选抗病关键生物学过程和抗病相关基因提供了新的思路。
Identification of disease-resistant genes and their application for disease-resistance breeding is an important way to solve disease problems in shrimp aquaculture. Although some transcriptomics studies have been performed to identify genes related to pathogen infection and host defense, there is still a lack of deep understanding of the host-resistant characteristics against V. parahaemolyticus infection. The present study established a method to obtain hemocytes from shrimp with clear resistant abilities against V. parahaemolyticus infection. We also proposed a strategy to identify key biological processes and genes related to disease resistance based on the analysis of the present transcriptome data. Vibrio parahaemolyticus carrying plasmid encoding toxins PirA and PirB is one of the causative agents leading to the severe disease of AHPND in shrimp aquaculture. However, there is a lack of deep understanding of the host-resistant characteristics against V. parahaemolyticus infection. Here, we established a method to obtain hemocytes from shrimp with different V. parahaemolyticus-resistant abilities and performed comparative transcriptome analysis on the expression profiles at the background level of hemocytes from shrimp in two independent populations. Principal component analysis and sample clustering results showed that samples from the same population had a closer relationship than that from shrimp with similar disease-resistant abilities. DEGs analysis revealed that the number of DEGs between two populations was much more than that between V. parahaemolyticus-resistant and susceptible shrimp. A total of 31 DEGs and 5 DEGs were identified from the comparison between V. parahaemolyticus-resistant and susceptible shrimp from populations 1 and 2, respectively. DEGs from population 1 were mainly cytoskeleton-related genes, metabolic related genes, and immune related genes. Although there was no DEGs overlap between two comparisons, DEGs from population 2 also included genes related to cytoskeleton and metabolism. The data suggest that these biological processes play important roles in disease resistance, and they could be focused by comprehensive analysis of multiple omics data. A new strategy for screening key biological processes and genes related to disease resistance was proposed based on the present study.
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影响因子：
7.3
作者：
Mai HN;Caro LFA;Cruz-Flores R;White BN;Dhar AK
通讯作者：
Dhar AK
DOI：
10.1128/genomea.00816-14
发表时间：
2014-09-04
期刊：
Genome announcements
影响因子：
--
作者：
Yang YT;Chen IT;Lee CT;Chen CY;Lin SS;Hor LI;Tseng TC;Huang YT;Sritunyalucksana K;Thitamadee S;Wang HC;Lo CF
通讯作者：
Lo CF
引起虾 AHPND 的质粒编码由 pirAB-Tn903 介导的 PirAB 毒素，在各种弧菌物种中普遍存在
影响因子：
4.6
作者：
Xiao J;Liu L;Ke Y;Li X;Liu Y;Pan Y;Yan S;Wang Y
通讯作者：
Wang Y
影响因子：
3.7
作者：
Velazquez-Lizarraga, Adrian E.;Luis Juarez-Morales, Jose;Ascencio, Felipe
通讯作者：
Ascencio, Felipe
影响因子：
5.9
作者：
Li J;Shen Y;Chen Y;Zhang Z;Ma S;Wan Q;Tong Q;Glaubitz C;Liu M;Yang J
通讯作者：
Yang J