Differentially Expressed Genes in Hepatopancreas of Acute Hepatopancreatic Necrosis Disease Tolerant and Susceptible Shrimp (Penaeus vannamei).
Differentially Expressed Genes in Hepatopancreas of Acute Hepatopancreatic Necrosis Disease Tolerant and Susceptible Shrimp (Penaeus vannamei).
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DOI：
10.3389/fimmu.2021.634152
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发表时间：
2021
影响因子：
7.3
通讯作者：
Dhar AK
中科院分区：
文献类型：
--
作者：
Mai HN;Caro LFA;Cruz-Flores R;White BN;Dhar AK
急性肝胰腺坏死病（Acute hepatopancreatic necrosis disease，AHPND）是一种严重危害亚洲和美洲对虾养殖业的致死性疾病。病原体是在质粒DNA中携带双毒素基因pirA和pirB的致病性弧菌。培育耐AHPND的对虾品系是防治该病的重要途径之一。在对南美白对虾进行抗病性筛选的过程中，发现一个选择的南美白对虾遗传品系对AHPND具有耐受性。通过定量RT-PCR测量了两个虾种群（即对AHPND敏感的P1和对AHPND耐受的P2）中已知参与细菌发病机制的12个免疫和代谢基因的mRNA表达。在这些基因中，胰凝乳蛋白酶A（ChyA）和丝氨酸蛋白酶（SP），参与代谢的基因，和甲壳素-P（CRSTP）和多酚氧化酶激活系统2（PPAE 2），参与细菌致病性的基因，在两个群体之间的mRNA表达差异。这些基因的差异表达揭示了凡纳滨对虾对AHPND的耐受机制，这些基因可能作为凡纳滨对虾对AHPND耐受/易感性的候选标记。这是首次报道的比较AHPND耐受和敏感的凡纳滨对虾品系的mRNA表达谱。
Acute hepatopancreatic necrosis disease (AHPND) is a lethal disease in marine shrimp that has caused large-scale mortalities in shrimp aquaculture in Asia and the Americas. The etiologic agent is a pathogenic Vibrio sp. carrying binary toxin genes, pirA and pirB in plasmid DNA. Developing AHPND tolerant shrimp lines is one of the prophylactic approaches to combat this disease. A selected genetic line of Penaeus vannamei was found to be tolerant to AHPND during screening for disease resistance. The mRNA expression of twelve immune and metabolic genes known to be involved in bacterial pathogenesis were measured by quantitative RT-PCR in two populations of shrimp, namely P1 that showed susceptibility to AHPND, and P2 that showed tolerance to AHPND. Among these genes, the mRNA expression of chymotrypsin A (ChyA) and serine protease (SP), genes that are involved in metabolism, and crustin-P (CRSTP) and prophenol oxidase activation system 2 (PPAE2), genes involved in bacterial pathogenesis in shrimp, showed differential expression between the two populations. The differential expression of these genes shed light on the mechanism of tolerance against AHPND and these genes can potentially serve as candidate markers for tolerance/susceptibility to AHPND in P. vannamei. This is the first report of a comparison of the mRNA expression profiles of AHPND tolerant and susceptible lines of P. vannamei.
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影响因子：
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