MCM2--a promising marker for premalignant lesions of the lung: a cohort study.
MCM2--a promising marker for premalignant lesions of the lung: a cohort study.
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DOI：
10.1186/1471-2407-1-6
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发表时间：
2001
期刊：
影响因子：
3.8
通讯作者：
Bepler G
中科院分区：
文献类型：
--
作者：
Tan DF;Huberman JA;Hyland A;Loewen GM;Brooks JS;Beck AF;Todorov IT;Bepler G
因为细胞进展为癌症必须增殖，特异于增殖细胞的标记蛋白可能允许检测癌前病变。在这里，我们比较了经典增殖标志物Ki-67与新的增殖标志物MCM2在41例支气管活检标本中的敏感性，这些标本分别代表了正常粘膜、化生、不典型增生和原位癌。平行切片用MCM2和Ki-67抗体染色，染色频率由两位研究者独立测量。采用双侧相关样本t检验和Wilcoxon秩和检验对差异进行统计评价。在41个标本中，抗mcm2染色的平均频率（39%）显著（p < 0.001）高于抗ki -67染色的平均频率（16%）。在化生病变中，抗mcm2经常检测到上皮表面附近的细胞，而抗ki -67则没有。我们得出结论，MCM2在增殖的癌前肺细胞中的检出率是Ki-67的2-3倍。MCM2作为癌前肺细胞的敏感标记物的前景被增强，因为它存在于化生肺病变表面的细胞中，这些细胞更有可能脱落到痰中。未来的研究将确定使用抗mcm2是否有可能足够早地发现肺癌，从而显著提高肺癌存活率。
Because cells progressing to cancer must proliferate, marker proteins specific to proliferating cells may permit detection of premalignant lesions. Here we compared the sensitivities of a classic proliferation marker, Ki-67, with a new proliferation marker, MCM2, in 41 bronchial biopsy specimens representing normal mucosa, metaplasia, dysplasia, and carcinoma in situ. Parallel sections were stained with antibodies against MCM2 and Ki-67, and the frequencies of staining were independently measured by two investigators. Differences were evaluated statistically using the two-sided correlated samples t-test and Wilcoxon rank sum test. For each of the 41 specimens, the average frequency of staining by anti-MCM2 (39%) was significantly (p < 0.001) greater than by anti-Ki-67 (16%). In metaplastic lesions anti-MCM2 frequently detected cells near the epithelial surface, while anti-Ki-67 did not. We conclude that MCM2 is detectable in 2-3 times more proliferating premalignant lung cells than is Ki-67. The promise of MCM2 as a sensitive marker for premalignant lung cells is enhanced by the fact that it is present in cells at the surface of metaplastic lung lesions, which are more likely to be exfoliated into sputum. Future studies will determine if use of anti-MCM2 makes possible sufficiently early detection to significantly enhance lung cancer survival rates.
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影响因子：
5.3
作者：
Bechtel, JJ;Petty, TL;Saccomanno, G
通讯作者：
Saccomanno, G
抗体KI-67的细胞增殖相关抗原：一种非常大的，无处不在的核蛋白，具有许多重复的元素，代表了一种新型的细胞周期维护蛋白。
影响因子：
7.8
作者：
Schluter, C;Duchrow, M;Wohlenberg, C;Becker, M H;Key, G;Flad, H D;Gerdes, J
通讯作者：
Gerdes, J
DOI：
10.1097/00043764-198608000-00038
发表时间：
1986-08-01
影响因子：
3.2
作者：
FONTANA, RS;SANDERSON, DR;MUHM, JR
通讯作者：
MUHM, JR
影响因子：
45.3
作者：
TOCKMAN, MS;GUPTA, PK;MULSHINE, JL
通讯作者：
MULSHINE, JL
影响因子：
9.6
作者：
Mountain, CF
通讯作者：
Mountain, CF