Endosome-mitochondria interactions are modulated by iron release from transferrin.
Endosome-mitochondria interactions are modulated by iron release from transferrin.
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DOI：
10.1083/jcb.201602069
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发表时间：
2016-09-26
期刊：
影响因子：
--
通讯作者：
Barroso MM
中科院分区：
文献类型：
--
作者：
Das A;Nag S;Mason AB;Barroso MM
Das等人利用超分辨率和定量荧光显微镜发现，含铁转铁蛋白的核内体直接与线粒体相互作用，促进上皮细胞中的铁转移。他们的发现进一步丰富了细胞器-细胞器直接相互作用的曲目，以实现功能意义。含有铁结合转铁蛋白（Tf）的核内体与线粒体之间的短暂“亲和运行”相互作用已被证明可促进红系细胞中铁的直接转移。在这项研究中，我们使用超分辨率三维（3D）直接随机光学重建显微镜来显示上皮细胞中含有tf的核内体直接与线粒体相互作用。我们使用活细胞延时荧光显微镜，然后是3D渲染，目标跟踪和距离转换算法，来跟踪tf内体并表征它们与线粒体相互作用的动力学。铁传感器rda标记的线粒体的猝灭证实了tf核内体相互作用的功能性铁转移。在与线粒体相互作用时，tf内体的运动性显著降低。为了进一步评估铁在Tf内体与线粒体相互作用能力中的功能作用，我们使用Tf K206E/K534A突变体阻断了内体铁的释放。阻断内体铁释放可显著增加tf -内体的运动性，延长内体-线粒体相互作用的持续时间。因此，内体铁调节了上皮细胞中含tf的内体和线粒体之间相互作用的动力学。
Using superresolution and quantitative fluorescence microscopy, Das et al. have revealed that iron-transferrin–containing endosomes directly interact with mitochondria, facilitating iron transfer in epithelial cells. Their findings further enrich the repertoire of organelle–organelle direct interactions to accomplish a functional significance. Transient “kiss and run” interactions between endosomes containing iron-bound transferrin (Tf) and mitochondria have been shown to facilitate direct iron transfer in erythroid cells. In this study, we used superresolution three-dimensional (3D) direct stochastic optical reconstruction microscopy to show that Tf-containing endosomes directly interact with mitochondria in epithelial cells. We used live-cell time-lapse fluorescence microscopy, followed by 3D rendering, object tracking, and a distance transformation algorithm, to track Tf-endosomes and characterize the dynamics of their interactions with mitochondria. Quenching of iron sensor RDA-labeled mitochondria confirmed functional iron transfer by an interacting Tf-endosome. The motility of Tf-endosomes is significantly reduced upon interaction with mitochondria. To further assess the functional role of iron in the ability of Tf-endosomes to interact with mitochondria, we blocked endosomal iron release by using a Tf K206E/K534A mutant. Blocking intraendosomal iron release led to significantly increased motility of Tf-endosomes and increased duration of endosome–mitochondria interactions. Thus, intraendosomal iron regulates the kinetics of the interactions between Tf-containing endosomes and mitochondria in epithelial cells.
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影响因子：
2.5
作者：
Che DL;Chowdary PD;Cui B
通讯作者：
Cui B
DOI：
10.1083/jcb.201505086
发表时间：
2015-12-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Guimaraes SC;Schuster M;Bielska E;Dagdas G;Kilaru S;Meadows BR;Schrader M;Steinberg G
通讯作者：
Steinberg G
影响因子：
4
作者：
Bouvet, Samuel;Golinelli-Cohen, Marie-Pierre;Jackson, Catherine L.
通讯作者：
Jackson, Catherine L.
影响因子：
7.2
作者：
Gautreau, Alexis;Oguievetskaia, Ksenia;Ungermann, Christian
通讯作者：
Ungermann, Christian
影响因子：
2.9
作者：
Halbrooks, PJ;He, QY;Mason, AB
通讯作者：
Mason, AB