Brownie, a gene involved in building complex respiratory devices in insect eggshells.
Brownie, a gene involved in building complex respiratory devices in insect eggshells.
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DOI：
10.1371/journal.pone.0008353
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发表时间：
2009-12-16
期刊：
影响因子：
3.7
通讯作者：
Piulachs MD
中科院分区：
文献类型：
--
作者：
Irles P;Bellés X;Piulachs MD
昆虫蛋壳必须将对卵黄和胚胎的保护与呼吸和精子进入的规定结合起来，这分别由气孔和珠孔来确保。将卵产在卵盒中的昆虫存在双重呼吸问题，因为气体交换涉及两个障碍。这种情况的一个例子是在德国蟑螂小蠊中发现的，其中的气孔和珠孔组合成一个称为海绵状体的复杂结构。这种海绵状的身体在形态上已经得到了很好的描述，但对于它是如何形成的却一无所知。在一个旨在寻找 B. germanica 晚期绒毛膜形成过程中表达的基因的文库中，我们分离出了新的序列 Bg30009（现在称为 Brownie），该序列因其高拷贝数而引人注目。在目前的工作中，我们发现布朗尼在绒毛膜形成晚期位于卵母细胞前极的滤泡细胞中表达。布朗尼的 RNA 干扰 (RNAi) 损害了海绵状体的正确形成，因此，蛋壳也形成不良，鸡蛋无法存活。结果表明，新基因布朗尼在海绵状身体的形成过程中发挥着关键作用。 Brownie 是第一个报道的参与复杂蛋壳呼吸结构构建的基因。
Insect eggshells must combine protection for the yolk and embryo with provisions for respiration and for the entry of sperm, which are ensured by aeropyles and micropyles, respectively. Insects which oviposit the eggs in an egg-case have a double problem of respiration as gas exchange then involves two barriers. An example of this situation is found in the cockroach Blattella germanica, where the aeropyle and the micropyle are combined in a complex structure called the sponge-like body. The sponge-like body has been well described morphologically, but nothing is known about how it is built up. In a library designed to find genes expressed during late chorion formation in B. germanica, we isolated the novel sequence Bg30009 (now called Brownie), which was outstanding due to its high copy number. In the present work, we show that Brownie is expressed in the follicle cells localized in the anterior pole of the oocyte in late choriogenesis. RNA interference (RNAi) of Brownie impaired correct formation of the sponge-like body and, as a result, the egg-case was also ill-formed and the eggs were not viable. Results indicate that the novel gene Brownie plays a pivotal role in building up the sponge-like body. Brownie is the first reported gene involved in the construction of complex eggshell respiratory structures.
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