Ultrastructural relationship between N-methyl-D-aspartate-NR1 receptor subunit and mu-opioid receptor in the mouse central nucleus of the amygdala.
Ultrastructural relationship between N-methyl-D-aspartate-NR1 receptor subunit and mu-opioid receptor in the mouse central nucleus of the amygdala.
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DOI：
10.1016/j.neuroscience.2009.07.020
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发表时间：
2009-10-20
期刊：
影响因子：
3.3
通讯作者：
Pickel, V. M.
中科院分区：
文献类型：
--
作者：
Glass, M. J.;Vanyo, L.;Quimson, L.;Pickel, V. M.
杏仁核中央核（CeA）是情绪过程的重要神经解剖学基底，情绪过程与成瘾行为密切相关。CeA中的谷氨酸和阿片系统在神经可塑性和成瘾过程中起重要作用，但N-甲基-d-天冬氨酸（NMDA）激动剂与CeA中μ-阿片受体（μOR）的相互作用的细胞位点尚不清楚。采用免疫细胞化学双标法观察NMDA-NR 1受体亚单位与μOR的超微结构关系。结果发现，超过80%的NR 1标记的配置文件是树突，而不到10%的轴突。在μ OR标记的情况下，大约60%是树突，超过35%是轴突。尽管它们的细胞定位模式有些不同，但仍观察到许多双标记谱。其中大约80%是树突状的，不到10%是轴突的。此外，许多双标记的树突状轮廓接触轴突终端接收非对称型突触兴奋性信号的指示。这些结果表明，NMDA和μORs的战略定位在树突，包括那些接受兴奋性突触，中央杏仁核神经元。因此，中央杏仁核神经元的突触后共调节可能是介导谷氨酸和阿片类药物对神经信号传导和可塑性的相互作用的关键细胞底物，这些神经信号传导和可塑性与成瘾行为相关的正常和病理性情绪过程相关。
The central nucleus of the amygdala (CeA) is an important neuroanatomical substrate of emotional processes that are critically involved in addictive behaviors. Glutamate and opioid systems in the CeA play significant roles in neural plasticity and addictive processes, however the cellular sites of interaction between agonists of N-methyl-d-aspar-tate (NMDA) and μ-opioid receptors (μOR) in the CeA are unknown. Dual labeling immunocytochemistry was used to determine the ultrastructural relationship between the essential NMDA-NR1 receptor subunit and μOR in the CeA. It was found that over 80% of NR1-labeled profiles were dendrites while less than 10% were axons. In the case of μOR-labeled profiles, approximately 60% were dendritic, and over 35% were axons. Despite their somewhat distinctive patterns of cellular location, numerous dual-labeled profiles were observed. Approximately 80% of these were dendritic, and less than 10% were axonal. Moreover, many dual-labeled dendritic profiles were contacted by axon terminals receiving asymmetric-type synapses indicative of excitatory signaling. These results indicate that NMDA and μORs are strategically localized in dendrites, including those receiving excitatory synapses, of central amygdala neurons. Thus, postsynaptic co-modulation of central amygdala neurons may be a key cellular substrate mediating glutamate and opioid interaction on neural signaling and plasticity associated with normal and pathological emotional processes associated with addictive behaviors.
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