Interactions between laminin receptor and the cytoskeleton during translation and cell motility.
Interactions between laminin receptor and the cytoskeleton during translation and cell motility.
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DOI：
10.1371/journal.pone.0015895
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发表时间：
2011-01-07
期刊：
影响因子：
3.7
通讯作者：
Meruelo D
中科院分区：
文献类型：
--
作者：
Venticinque L;Jamieson KV;Meruelo D
人层粘连蛋白受体既作为40 S核糖体亚基的组分介导细胞翻译，又作为与细胞外基质组分相互作用的细胞表面受体。由于其作为几种病毒的细胞表面受体的作用及其在几种类型的癌症中的过表达，层粘连蛋白受体是一种病理学上重要的蛋白质。先前的研究已经确定核糖体与细胞骨架的组分相关，但是负责的特定核糖体组分尚未确定。我们的研究表明层粘连蛋白受体直接与微管蛋白结合。通过使用siRNA和细胞骨架抑制剂，我们证明了层粘连蛋白受体作为一种束缚蛋白，将核糖体固定在微管蛋白上，微管蛋白是细胞翻译的组成部分。我们的研究还表明层粘连蛋白受体能够直接与肌动蛋白结合。通过使用siRNA和细胞骨架抑制剂，我们已经表明，这种层粘连蛋白受体-肌动蛋白相互作用是细胞迁移的关键。这些数据表明，层粘连蛋白受体和细胞骨架之间的相互作用在介导与癌症密切相关的两个过程，细胞翻译和迁移中至关重要。
Human laminin receptor acts as both a component of the 40S ribosomal subunit to mediate cellular translation and as a cell surface receptor that interacts with components of the extracellular matrix. Due to its role as the cell surface receptor for several viruses and its overexpression in several types of cancer, laminin receptor is a pathologically significant protein. Previous studies have determined that ribosomes are associated with components of the cytoskeleton, however the specific ribosomal component(s) responsible has not been determined. Our studies show that laminin receptor binds directly to tubulin. Through the use of siRNA and cytoskeletal inhibitors we demonstrate that laminin receptor acts as a tethering protein, holding the ribosome to tubulin, which is integral to cellular translation. Our studies also show that laminin receptor is capable of binding directly to actin. Through the use of siRNA and cytoskeletal inhibitors we have shown that this laminin receptor-actin interaction is critical for cell migration. These data indicate that interactions between laminin receptor and the cytoskeleton are vital in mediating two processes that are intimately linked to cancer, cellular translation and migration.
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