The flavonoid, naringenin, decreases adipose tissue mass and attenuates ovariectomy-associated metabolic disturbances in mice.
The flavonoid, naringenin, decreases adipose tissue mass and attenuates ovariectomy-associated metabolic disturbances in mice.
复制标题
DOI：
10.1186/1743-7075-12-1
复制
发表时间：
2015
影响因子：
4.5
通讯作者：
Belury MA
中科院分区：
文献类型：
--
作者：
Ke JY;Kliewer KL;Hamad EM;Cole RM;Powell KA;Andridge RR;Straka SR;Yee LD;Belury MA
与卵巢功能丧失相关的不良代谢变化增加了绝经后妇女发生代谢综合征和非酒精性脂肪性肝病（NAFLD）的风险。柚皮素改善体外和体内代谢紊乱。在本研究中，我们测试了柚皮素对卵巢切除小鼠雌激素缺乏引起的代谢紊乱的影响。切除卵巢的C57 BL/6 J雌性小鼠喂食对照饮食（10%来自脂肪的卡路里）11周。在接下来的11周内，小鼠继续对照饮食（n = 9）或转换为补充有3%柚皮素的对照饮食（n = 10）。通过间接量热法测量能量消耗，并通过红外光束中断监测活性。通过磁共振成像（MRI）评价腹腔内和皮下脂肪。代谢反应的血液生化指标包括葡萄糖、胰岛素、脂肪因子和脂质。定量肝脏和肌肉中的脂质含量以及脂肪组织、肝脏和肌肉中相关基因的表达。与对照组相比，喂食柚皮素的卵巢切除小鼠表现出较低的空腹血糖和胰岛素水平，腹腔内和皮下脂肪减少超过50%。血浆瘦素和瘦素mRNA的脂肪库也减少了小鼠喂食柚皮素饮食。单核细胞趋化蛋白-1（MCP 1/CCl 2）和白细胞介素6（IL-6/IL 6）mRNA表达水平显着降低，在周围脂肪组织的柚皮素补充小鼠。我们还观察到，喂食柚皮素饮食的小鼠具有较少的肝脏脂质蓄积，相应地改变了与从头脂肪生成、脂肪酸氧化和脂肪生成相关的肝脏基因表达。膳食中的柚皮素减轻了雌性小鼠卵巢切除术相关的许多代谢紊乱。本文的在线版本（doi：10.1186/1743-7075-12-1）包含补充材料，可供授权用户使用。
Adverse metabolic changes associated with loss of ovarian function increase the risk of developing metabolic syndrome and non-alcoholic fatty liver disease (NAFLD) in postmenopausal women. Naringenin improves metabolic disturbances in vitro and in vivo. In the present study, we tested the effects of naringenin on metabolic disturbances resulting from estrogen deficiency in ovariectomized mice. Ovariectomized C57BL/6 J female mice were fed a control diet (10% calories from fat) for 11 weeks. Mice either continued on the control diet (n = 9) or were switched to the control diet supplemented with 3% naringenin (n = 10) for the next 11 weeks. Energy expenditure was measured by indirect calorimetry and activity was monitored by infrared beam breaks. Intra-abdominal and subcutaneous adiposity was evaluated by magnetic resonance imaging (MRI). Blood biochemical measures of metabolic response included glucose, insulin, adipokines, and lipids. Lipid content in liver and muscle and expression of relevant genes in adipose tissue, liver, and muscle were quantified. Ovariectomized mice fed naringenin exhibited lower fasting glucose and insulin levels compared to controls, with over 50% reduction of intra-abdominal and subcutaneous adiposity. Plasma leptin and leptin mRNA in adipose depots were also decreased in mice fed a naringenin diet. Monocyte chemoattractant protein-1 (MCP1/Ccl2) and interleukin 6 (IL-6/Il6) mRNA expression levels were significantly lower in perigonadal adipose tissue of naringenin-supplemented mice. We also observed that mice fed a naringenin diet had less hepatic lipid accumulation with corresponding alterations of hepatic gene expression associated with de novo lipogenesis, fatty acid oxidation, and gluconeogenesis. Dietary naringenin attenuates many of the metabolic disturbances associated with ovariectomy in female mice. The online version of this article (doi:10.1186/1743-7075-12-1) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
2.2
作者：
Lavoie JM;Pighon A
通讯作者：
Pighon A
影响因子：
4.9
作者：
Lovejoy, J. C.;Champagne, C. M.;Smith, S. R.
通讯作者：
Smith, S. R.
影响因子：
3.7
作者：
Goldwasser J;Cohen PY;Yang E;Balaguer P;Yarmush ML;Nahmias Y
通讯作者：
Nahmias Y
影响因子：
7.7
作者：
Borradaile, NM;de Dreu, LE;Huff, MW
通讯作者：
Huff, MW
影响因子：
4.8
作者：
D'Eon, TM;Souza, SC;Greenberg, AS
通讯作者：
Greenberg, AS