High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device.
High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device.
复制标题
DOI：
10.1371/journal.pone.0033876
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Abe K
中科院分区：
文献类型：
--
作者：
Cao L;Kobayakawa S;Yoshiki A;Abe K
具有亚细胞分辨率的颅骨中脑和骨髓细胞的活体成像彻底改变了神经生物学、免疫学和血液学。然而，这项强大技术在腹部器官研究中的应用长期以来一直受到呼吸和心跳引起的器官运动的阻碍。在这里，我们首次描述了一种被称为“微型载物台”的简单装置，它可以有效减少器官运动而不引起组织损伤。该系统将该微型载物台装置与配备独特棒型物镜的直立式活体激光扫描显微镜相结合，能够对活体小鼠腹部器官进行亚细胞水平成像。我们证明，该技术可以定量分析细胞中的亚细胞结构和基因表达，实时跟踪细胞内过程以及活体小鼠胰腺和肝脏的三维图像构建。由于上述基于亚细胞成像的分析可以扩展到其他腹膜内器官，因此该技术为研究腹部器官的生理和疾病特异性事件提供了巨大的潜力。微载物台方法为体内成像工具箱增添了一项令人兴奋的新技术。
Intravital imaging of brain and bone marrow cells in the skull with subcellular resolution has revolutionized neurobiology, immunology and hematology. However, the application of this powerful technology in studies of abdominal organs has long been impeded by organ motion caused by breathing and heartbeat. Here we describe for the first time a simple device designated ‘microstage’ that effectively reduces organ motions without causing tissue lesions. Combining this microstage device with an upright intravital laser scanning microscope equipped with a unique stick-type objective lens, the system enables subcellular-level imaging of abdominal organs in live mice. We demonstrate that this technique allows for the quantitative analysis of subcellular structures and gene expressions in cells, the tracking of intracellular processes in real-time as well as three-dimensional image construction in the pancreas and liver of the live mouse. As the aforementioned analyses based on subcellular imaging could be extended to other intraperitoneal organs, the technique should offer great potential for investigation of physiological and disease-specific events of abdominal organs. The microstage approach adds an exciting new technique to the in vivo imaging toolbox.
登录
查看更多内容
影响因子：
48
作者：
Drew, Patrick J.;Shih, Andy Y.;Driscoll, Jonathan D.;Knutsen, Per Magne;Blinder, Pablo;Davalos, Dimitrios;Akassoglou, Katerina;Tsai, Philbert S.;Kleinfeld, David
通讯作者：
Kleinfeld, David
影响因子：
56.9
作者：
Nimmerjahn, A;Kirchhoff, F;Helmchen, F
通讯作者：
Helmchen, F
影响因子：
15.9
作者：
Nyman, Lara R.;Wells, K. Sam;Powers, Alvin C.
通讯作者：
Powers, Alvin C.
影响因子：
82.9
作者：
Iwawaki, T;Akai, R;Miura, M
通讯作者：
Miura, M
影响因子：
11.2
作者：
Serrels, Alan;Timpson, Paul;Anderson, Kurt I.
通讯作者：
Anderson, Kurt I.