BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism.
BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism.
复制标题
BAC 和 RNA 测序揭示重组冷点中的褐飞虱抗性基因 BPH15 介导独特的防御机制
DOI：
10.1186/1471-2164-15-674
复制
发表时间：
2014-08-11
期刊：
影响因子：
4.4
通讯作者：
He G
中科院分区：
文献类型：
--
作者：
Lv W;Du B;Shangguan X;Zhao Y;Pan Y;Zhu L;He Y;He G
褐飞虱（Nilaparvata lugens Stål）是水稻上危害性最大的食韧皮部害虫。抗稻瘟病菌基因BPH 15已被证明是有效的控制害虫，并广泛应用于水稻育种计划。然而，耐药的分子机制仍不清楚。在本研究中，我们缩小了BPH 15在4号染色体上的位置，并研究了BPH 15水稻在褐飞虱攻击后的转录组。以感病水稻TN 1和携带BPH 15基因的重组自交系RI 93为亲本，对13,000株BC 2F 2代植株进行了分析。BPH 15定位于4号染色体上的0.0269 cM区域，该区域在日本晴的参考基因组中为210-kb。对跨越BPH 15区域的细菌人工染色体（BAC）克隆进行测序，结果表明，抗病水稻B5的BPH 15区域的物理大小为580-kb，远大于日本晴参考基因组中的相应区域。抗病水稻BPH 15区有87个预测基因。分析预测基因的表达谱。结果表明，4个jacalin相关凝集素基因和1个LRR蛋白基因在抗病亲本中组成型表达，可能是BPH 15的候选基因。利用高通量RNA测序技术对BPH 15抗性导入系和感病受体系的转录组进行了分析。总共鉴定了2，914个差异表达基因（DEG）。抗虫和易感植物之间以及早期（侵染后6小时，HAI）和晚期（48 HAI）之间的BPH响应转录谱不同。主要的防御机制与茉莉酸信号、乙烯信号、受体激酶、MAPK级联、Ca 2+信号、PR基因、转录因子和蛋白质翻译后修饰有关。我们的工作结合BAC和RNA测序来鉴定BPH 15的候选基因，并揭示其介导的抗性机制。这些结果增加了我们对植物-昆虫相互作用的理解，并可用于预防这种破坏性的农业害虫。本文的在线版本（doi：10.1186/1471-2164-15-674）包含补充材料，可供授权用户使用。
Brown planthopper (BPH, Nilaparvata lugens Stål), is the most destructive phloem-feeding insect pest of rice (Oryza sativa). The BPH-resistance gene BPH15 has been proved to be effective in controlling the pest and widely applied in rice breeding programs. Nevertheless, molecular mechanism of the resistance remain unclear. In this study, we narrowed down the position of BPH15 on chromosome 4 and investigated the transcriptome of BPH15 rice after BPH attacked. We analyzed 13,000 BC2F2 plants of cross between susceptible rice TN1 and the recombinant inbred line RI93 that carrying the BPH15 gene from original resistant donor B5. BPH15 was mapped to a 0.0269 cM region on chromosome 4, which is 210-kb in the reference genome of Nipponbare. Sequencing bacterial artificial chromosome (BAC) clones that span the BPH15 region revealed that the physical size of BPH15 region in resistant rice B5 is 580-kb, much bigger than the corresponding region in the reference genome of Nipponbare. There were 87 predicted genes in the BPH15 region in resistant rice. The expression profiles of predicted genes were analyzed. Four jacalin-related lectin proteins genes and one LRR protein gene were found constitutively expressed in resistant parent and considered the candidate genes of BPH15. The transcriptomes of resistant BPH15 introgression line and the susceptible recipient line were analyzed using high-throughput RNA sequencing. In total, 2,914 differentially expressed genes (DEGs) were identified. BPH-responsive transcript profiles were distinct between resistant and susceptible plants and between the early stage (6 h after infestation, HAI) and late stage (48 HAI). The key defense mechanism was related to jasmonate signaling, ethylene signaling, receptor kinase, MAPK cascades, Ca2+ signaling, PR genes, transcription factors, and protein posttranslational modifications. Our work combined BAC and RNA sequencing to identify candidate genes of BPH15 and revealed the resistance mechanism that it mediated. These results increase our understanding of plant–insect interactions and can be used to protect against this destructive agricultural pest. The online version of this article (doi:10.1186/1471-2164-15-674) contains supplementary material, which is available to authorized users.
登录
查看更多内容
DOI：
10.1007/s004380050660
发表时间：
1998-02-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Kaloshian, I;Yaghoobi, J;Williamson, VM
通讯作者：
Williamson, VM
影响因子：
7.4
作者：
Hao, Peiying;Liu, Caixiang;He, Guangcun
通讯作者：
He, Guangcun
影响因子：
4.1
作者：
Hu, Jie;Cheng, Mingxing;He, Yuqing
通讯作者：
He, Yuqing
影响因子：
5.1
作者：
Koo, Yeon Jong;Kim, Myeong Ae;Do Choi, Yang
通讯作者：
Do Choi, Yang
影响因子：
3.3
作者：
Paape T;Zhou P;Branca A;Briskine R;Young N;Tiffin P
通讯作者：
Tiffin P